000000615 001__ 615
000000615 005__ 20260106151648.0
000000615 0247_ $$2DOI$$a10.6083/M4D50JZF
000000615 037__ $$aETD
000000615 245__ $$aApplication of molecular biological tools to the study of bioremediation in contaminated sediments
000000615 260__ $$bOregon Health and Science University
000000615 269__ $$a2011
000000615 336__ $$aDissertation
000000615 502__ $$bPh.D.
000000615 502__ $$gEnvironmental Systems and Human Health
000000615 520__ $$aThis study assessed cryogenic preservation for molecular characterization of microorganisms in sediment using samples from a toluene-contaminated model aquifer. qPCR, RT-qPCR, and PCR-SSCP showed no significant differences between frozen and unfrozen sediments, validating cryogenic coring for accurate subsurface microbial analysis. Functional gene profiling revealed distinct sediment-attached versus suspended toluene-degrading communities, with sediment data better reflecting groundwater chemistry. Functional gene abundance and transcripts alone proved unreliable indicators of biodegradation due to basal expression. An integrated approach using the ratio of transcripts to gene copies is proposed as a more accurate measure of in-situ metabolic activity, requiring system-specific activity thresholds.
000000615 540__ $$fCC BY
000000615 542__ $$fIn copyright - single owner
000000615 650__ $$aGene Expression$$028717
000000615 650__ $$aWater Pollutants, Chemical$$027939
000000615 650__ $$aPolymerase Chain Reaction$$028906
000000615 650__ $$aPolymorphism, Single-Stranded Conformational$$031111
000000615 650__ $$aBiodegradation, Environmental$$015551
000000615 6531_ $$acontaminated sediments
000000615 6531_ $$abioremediation
000000615 691__ $$aSchool of Medicine$$041369
000000615 692__ $$aDepartment of Environmental and Biomolecular Systems$$041414
000000615 7001_ $$aBrow, Christina$$uOregon Health and Science University$$041354
000000615 7201_ $$aSimon, Holly$$uOregon Health and Science University$$041354$$7Personal$$eCo-Advisor
000000615 7201_ $$aJohnson, Richard$$uOregon Health and Science University$$041354$$7Personal$$eCo-Advisor
000000615 8564_ $$9977a6d05-b6eb-46b5-a978-e101d085dccb$$s2934505$$uhttps://digitalcollections.ohsu.edu/record/615/files/616_etd.pdf$$ePublic$$211f177a219e2246f4d2479440ffd393c$$31
000000615 905__ $$a/rest/prod/z3/16/q1/62/z316q162x
000000615 909CO $$ooai:digitalcollections.ohsu.edu:615$$pstudent-work
000000615 980__ $$aTheses and Dissertations