000000624 001__ 624 000000624 005__ 20260304130441.0 000000624 0247_ $$2DOI$$a10.6083/M47H1GJH 000000624 037__ $$aETD 000000624 245__ $$aWnt signaling in the mouse small intestine 000000624 260__ $$bOregon Health and Science University 000000624 269__ $$a2009 000000624 336__ $$aDissertation 000000624 502__ $$bPh.D. 000000624 520__ $$aWnt signaling is essential for maintaining the intestinal epithelium, promoting stem cell renewal while preventing excessive proliferation. Although 19 Wnt ligands exist in humans and mice, their individual roles in the intestine remain unclear. This work characterizes canonical Wnt‑activated cells in the adult intestine and examines interactions with non‑canonical ligands. Wnt‑reporter activity was confined to stem‑cell–rich crypt bases, largely excluding transient‑amplifying cells, and expanded following γ‑irradiation. Overexpression of WNT1 increased proliferation and induced polyp formation, underscoring the need for tight pathway control. Conversely, ectopic expression of the non‑canonical ligand Wnt5a inhibited canonical signaling and altered Paneth cell localization. Together, these findings highlight distinct ligand‑specific functions and reveal regulatory feedback mechanisms that shape intestinal homeostasis and regeneration. 000000624 540__ $$fCC BY 000000624 542__ $$fIn copyright - single owner 000000624 650__ $$aIntestines$$020963 000000624 650__ $$aMice$$036842 000000624 650__ $$aStem Cells$$026391 000000624 650__ $$aWnt Proteins$$036730 000000624 650__ $$aWnt Signaling Pathway$$039751 000000624 691__ $$aSchool of Medicine$$041369 000000624 692__ $$aDepartment of Molecular and Medical Genetics$$041428 000000624 7001_ $$aDismuke, Adria$$uOregon Health and Science University$$041354 000000624 7201_ $$aWong, Melissa$$uOregon Health and Science University$$041354$$7Personal$$eMentor 000000624 8564_ $$99740f149-7945-41f8-8572-8be972f0bad5$$s7165176$$uhttps://digitalcollections.ohsu.edu/record/624/files/625_etd.pdf$$ePublic$$27b38267f51f56cc3ae1ec6bb507acde6$$31 000000624 905__ $$a/rest/prod/wh/24/6s/20/wh246s20z 000000624 909CO $$ooai:digitalcollections.ohsu.edu:624$$pstudent-work 000000624 956__ $$aGet Accessible Copy$$uhttps://ohsu.libwizard.com/f/requestaccessibledocument 000000624 980__ $$aTheses and Dissertations