TY  - THES
AB  - Manganese oxidation plays a key role in global biogeochemical cycling, yet its cellular function and enzymatic mechanisms remain unclear. This study identifies MopA, a novel Mn(II)-oxidizing peroxidase in Aurantimonas manganoxydans SI85-9A1—the first bacterial peroxidase linked to Mn oxidation. Functional assays suggest involvement of heme peroxidase and reactive oxygen species, with superoxide mediating Mn(II→III) and hydrogen peroxide Mn(III→IV) oxidation. Development of a genetic system via plasmid incorporation marks a breakthrough for mechanistic studies. Findings indicate Mn oxidation involves multiple oxygen-dependent steps and a complex protein system, advancing understanding of microbial manganese metabolism.
AD  - Oregon Health and Science University
AU  - Caputo, Nicholas
DA  - 2011
DO  - 10.6083/M4X06510
DO  - DOI
ED  - Tebo, Bradley
ED  - Anderson, Craig
ED  - Advisor
ED  - Mentor
ID  - 642
KW  - Manganese
KW  - Peroxidases
KW  - Reactive Oxygen Species
KW  - Peroxidase
KW  - Oxidation-Reduction
KW  - Aurantimonas manganoxydans
KW  - oxidation-reduction reaction
L1  - https://digitalcollections.ohsu.edu/record/642/files/643_etd.pdf
L2  - https://digitalcollections.ohsu.edu/record/642/files/643_etd.pdf
L4  - https://digitalcollections.ohsu.edu/record/642/files/643_etd.pdf
LK  - https://digitalcollections.ohsu.edu/record/642/files/643_etd.pdf
N2  - Manganese oxidation plays a key role in global biogeochemical cycling, yet its cellular function and enzymatic mechanisms remain unclear. This study identifies MopA, a novel Mn(II)-oxidizing peroxidase in Aurantimonas manganoxydans SI85-9A1—the first bacterial peroxidase linked to Mn oxidation. Functional assays suggest involvement of heme peroxidase and reactive oxygen species, with superoxide mediating Mn(II→III) and hydrogen peroxide Mn(III→IV) oxidation. Development of a genetic system via plasmid incorporation marks a breakthrough for mechanistic studies. Findings indicate Mn oxidation involves multiple oxygen-dependent steps and a complex protein system, advancing understanding of microbial manganese metabolism.
PB  - Oregon Health and Science University
PY  - 2011
T1  - Investigating manganese oxidation by Aurantimonas manganoxydans S185-9A1
TI  - Investigating manganese oxidation by Aurantimonas manganoxydans S185-9A1
UR  - https://digitalcollections.ohsu.edu/record/642/files/643_etd.pdf
Y1  - 2011
ER  -