000000653 001__ 653
000000653 005__ 20260115072209.0
000000653 0247_ $$2DOI$$a10.6083/M4GT5K62
000000653 037__ $$aETD
000000653 245__ $$aThe effect of ischemia on cerebellar purkinje cell GABAA receptors
000000653 260__ $$bOregon Health and Science University
000000653 269__ $$a2011
000000653 336__ $$aDissertation
000000653 502__ $$bPh.D.
000000653 502__ $$gNeuroscience
000000653 520__ $$aPurkinje cells (PCs) are highly vulnerable to ischemic injury, yet the role of GABAergic signaling in cerebellar neuroprotection remains poorly defined. Using cerebellar cultures subjected to oxygen‑glucose deprivation and a mouse model of global ischemia, we found that ischemia rapidly reduces GABAA_AA​ receptor currents and protein levels in PCs while sparing β1‑containing receptors. The neurosteroid allopregnanolone (ALLO) prevented this ischemia‑induced decline and protected PCs both in vitro and in vivo. ALLO’s protective effects were more pronounced in females at low doses. These findings identify GABAA_AA​ receptor loss as a key contributor to PC vulnerability and highlight ALLO as a promising therapeutic candidate for ischemic cerebellar injury.
000000653 540__ $$fCC BY
000000653 542__ $$fIn copyright - single owner
000000653 650__ $$aSex Characteristics$$025917
000000653 650__ $$aPurkinje Cells$$024938
000000653 650__ $$aIschemia$$021046
000000653 650__ $$aNeuroprotection$$011086
000000653 650__ $$aPregnanolone$$024553
000000653 650__ $$aReceptors, GABA-A$$025198
000000653 6531_ $$aalloprenanolone
000000653 6531_ $$asex differences
000000653 691__ $$aSchool of Medicine$$041369
000000653 692__ $$aVollum Institute$$041509
000000653 7001_ $$aKelley, Melissa$$uOregon Health and Science University$$041354
000000653 7201_ $$aHerson, Paco$$uOregon Health and Science University$$041354$$7Personal$$eAdvisor
000000653 8564_ $$99a6e3869-3e98-4521-b86d-52e52a79189f$$s2097817$$uhttps://digitalcollections.ohsu.edu/record/653/files/654_etd.pdf$$ePublic$$245333b79c348fb48b8df0bab5fb8f043$$31
000000653 905__ $$a/rest/prod/31/97/xm/07/3197xm07c
000000653 909CO $$ooai:digitalcollections.ohsu.edu:653$$pstudent-work
000000653 956__ $$aGet Accessible Copy$$uhttps://ohsu.libwizard.com/f/requestaccessibledocument
000000653 980__ $$aTheses and Dissertations