000000694 001__ 694
000000694 005__ 20260120144837.0
000000694 0247_ $$2DOI$$a10.6083/M4668B6M
000000694 037__ $$aETD
000000694 245__ $$aStructure and function of the iron-regulatory protein, repulsive guidance molecule c / hemojuvelin
000000694 260__ $$bOregon Health and Science University
000000694 269__ $$a2011
000000694 336__ $$aDissertation
000000694 502__ $$bPh.D.
000000694 502__ $$gBiochemistry & Molecular Biology (sunsetting)
000000694 520__ $$aIron homeostasis depends on tightly regulated mechanisms that prevent both deficiency and toxic iron overload. Mutations in RGMc/HJV cause juvenile hemochromatosis, a severe early‑onset iron‑loading disorder marked by reduced hepcidin expression. This work investigates RGMc function using structure‑function analyses, revealing that full-length and soluble RGMc isoforms act as potent antagonists of BMP2‑ and BMP6‑mediated signaling. Using an MS3‑based disulfide mapping approach, we identified disulfide bonds in IGFBP‑5 and uncovered two distinct oxidation‑dependent disulfide patterns in RGMc. These findings provide new insight into RGMc structure and its role in iron regulation.
000000694 540__ $$fCC BY
000000694 542__ $$fIn copyright - single owner
000000694 650__ $$aRepressor Proteins$$025328
000000694 650__ $$aMass Spectrometry$$026224
000000694 650__ $$aMembrane Proteins$$022009
000000694 650__ $$aBone Morphogenetic Proteins$$031660
000000694 650__ $$aMuscle Proteins$$022527
000000694 650__ $$aInsulin-Like Growth Factor Binding Protein 5$$031248
000000694 650__ $$aIron Overload$$031414
000000694 650__ $$aHemochromatosis, type 2
000000694 6531_ $$ahemojuvelin
000000694 6531_ $$argmc
000000694 6531_ $$arepulsive guidance molecule c
000000694 691__ $$aSchool of Medicine$$041369
000000694 692__ $$aDepartment of Biochemistry and Molecular Biology$$041396
000000694 7001_ $$aNili, Mahta$$uOregon Health and Science University$$041354
000000694 7201_ $$aShinde, Ujwal$$uOregon Health and Science University$$041354$$7Personal$$eAdvisor
000000694 7201_ $$aRotwein, Peter$$uOregon Health and Science University$$041354$$7Personal$$eAdvisor
000000694 8564_ $$9517b12e9-ef89-476f-a477-20af968a63fb$$s3734077$$uhttps://digitalcollections.ohsu.edu/record/694/files/695_etd.pdf$$ePublic$$2948f20e75cebb0fc1994db0cc9efae7e$$31
000000694 905__ $$a/rest/prod/v9/79/v3/09/v979v309v
000000694 909CO $$ooai:digitalcollections.ohsu.edu:694$$pstudent-work
000000694 956__ $$aGet Accessible Copy$$uhttps://ohsu.libwizard.com/f/requestaccessibledocument
000000694 980__ $$aTheses and Dissertations