000007486 001__ 7486
000007486 005__ 20231129124939.0
000007486 0247_ $$2DOI$$a10.6083/2j62s534w
000007486 037__ $$aETD
000007486 245__ $$aHypermethylation in the promoter of PRDM16 is associated with decreased survival in AML patients
000007486 260__ $$bOregon Health and Science University
000007486 269__ $$a2019
000007486 336__ $$aThesis
000007486 502__ $$bM.S.
000007486 520__ $$aAcute Myeloid Leukemia (AML) is the most common acute adult leukemia, accounting for approximately 1% of all new cancers in 2018. DNA methylation is an epigenetic modification that has been shown to play a strong role in the development and progression of AML. Disruption of DNA methylation in patients is often due to somatic mutations of epigenetic modifier genes (e.g. (DNMT3A, TET2, IDH1/2, WT1, EZH2, ASXL1). Typically, studies of DNA methylation in AML and other cancers focus on regions of differential methylation (DMRs) between cancer patients and their healthy counterparts. Using the data from The Cancer Genome Atlas study on 194 AML patients, we investigated differential methylation between patients with and without mutations of the epigenetic modification genes commonly mutated in AML. We identified 20,567 differentially methylated probes within 2,893 DMRs and analyzed the affected regions for involvement in AML. In particular, we identified a region within the promoter of PRDM16 which, when hypermethylated, is associated with decreased survival (p = 0.017). In conclusion, we find DMRs between AML patients with and without somatic mutations of epigenetic modification genes that affect biologically relevant pathway and show association with the development and progression of AML.
000007486 650__ $$aLeukemia$$021430
000007486 650__ $$aGenes$$019447
000007486 650__ $$aDNA Methylation$$031400
000007486 650__ $$aHumans$$020376
000007486 650__ $$aAdult$$014319
000007486 6531_ $$aepigenesis
000007486 6531_ $$agenetic
000007486 6531_ $$aacute
000007486 6531_ $$amodifier
000007486 6531_ $$amyeloid
000007486 691__ $$aSchool of Medicine$$041369
000007486 7001_ $$aSmith, Connor
000007486 8564_ $$9b3dc33d1-5679-4390-9dd8-6b5dd21d3b86$$s1421148$$uhttps://digitalcollections.ohsu.edu/record/7486/files/smith.connor.2019.pdf
000007486 8564_ $$9021ac847-b4e3-4511-82be-36fa5e4c77c0$$s300758$$uhttps://digitalcollections.ohsu.edu/record/7486/files/smith.connor.SupplementaryData.2019.xlsx
000007486 8564_ $$9f1592c51-2985-46db-858c-f51729e24d16$$s415822$$uhttps://digitalcollections.ohsu.edu/record/7486/files/smith.connor.SupplementalFigures.2019.pdf
000007486 905__ $$a/rest/prod/2j/62/s5/34/2j62s534w
000007486 909CO $$ooai:digitalcollections.ohsu.edu:7486$$pstudent-work
000007486 980__ $$aTheses and Dissertations