TY  - GEN
AB  - This work examines the role of human cytomegalovirus (HCMV) envelope glycoproteins in viral entry. Using genetic and biochemical approaches, we show that gO acts as a molecular chaperone for gH/gL ER export but dissociates before virion assembly; deletion of gO reduces gH/gL incorporation and blocks entry into fibroblasts and epithelial/endothelial cells. We also demonstrate that gB functions as the fusion protein: cells expressing gB mediate entry of gB-deficient virions in trans, whereas gH/gL cannot. These findings support a model where gH/gL binds cellular receptors and interacts with gB to trigger membrane fusion during HCMV entry.
AD  - Oregon Health and Science University
AU  - Wille, Paul
DA  - 2011
DO  - 10.6083/M4PV6HC2
DO  - DOI
ED  - Johnson, David
ED  - Mentor
ID  - 750
KW  - Glycoproteins
KW  - Genetics
KW  - Cytomegalovirus
KW  - Membrane Glycoproteins
KW  - cytomegaloviruses
KW  - human cytomegalovirus
L1  - https://digitalcollections.ohsu.edu/record/750/files/753_etd.pdf
L2  - https://digitalcollections.ohsu.edu/record/750/files/753_etd.pdf
L4  - https://digitalcollections.ohsu.edu/record/750/files/753_etd.pdf
LK  - https://digitalcollections.ohsu.edu/record/750/files/753_etd.pdf
N2  - This work examines the role of human cytomegalovirus (HCMV) envelope glycoproteins in viral entry. Using genetic and biochemical approaches, we show that gO acts as a molecular chaperone for gH/gL ER export but dissociates before virion assembly; deletion of gO reduces gH/gL incorporation and blocks entry into fibroblasts and epithelial/endothelial cells. We also demonstrate that gB functions as the fusion protein: cells expressing gB mediate entry of gB-deficient virions in trans, whereas gH/gL cannot. These findings support a model where gH/gL binds cellular receptors and interacts with gB to trigger membrane fusion during HCMV entry.
PB  - Oregon Health and Science University
PY  - 2011
T1  - How human cytomaegalovirus glycoproteins mediate entry
TI  - How human cytomaegalovirus glycoproteins mediate entry
UR  - https://digitalcollections.ohsu.edu/record/750/files/753_etd.pdf
Y1  - 2011
ER  -