000007535 001__ 7535
000007535 005__ 20231129124938.0
000007535 0247_ $$2DOI$$a10.6083/2b88qc733
000007535 037__ $$aETD
000007535 245__ $$aA multi-modal investigation of the developmental origins of congenital heart defects in avian embryos
000007535 260__ $$bOregon Health and Science University
000007535 269__ $$a2019
000007535 336__ $$aThesis
000007535 502__ $$bM.S.
000007535 520__ $$aMaternal conditions that disrupt the normal embryonic environment during heart development can lead to structural defects in the heart, ultimately compromising the heart's function and leading to increased morbidity and mortality. Avian models of cardiovascular development have played a key role in elucidating the mechanisms by which congenital heart defects form during critical periods of early heart development. However, current avian models are restricted to narrow developmental windows, thereby limiting one's ability to study the manifestation of cardiac defects over a larger, more complete timescale. The work presented in this thesis uses novel techniques in an avian model coupled with multiscale imaging modalities to study how transient interventions during early heart formation affect the overall development of the heart. Specifically, we employ a combination of 3D electron microscopy, light microscopy, and micro-computed tomography to understand how pharmacological or surgical interventions performed during tubular heart stages affect both early- and late-stages of cardiac development. Overall, 
our studies revealed unreported cardiac malformation phenotypes in response to early interventions as well as accompanying ultrastructural changes in cardiac tissues. Furthermore, the models and methods presented here provide a framework for a comprehensive multiscale developmental analysis that can potentially be applied to other organs in the chick, and adapted for use in other small animal models.
000007535 650__ $$aPhenotype$$023948
000007535 650__ $$aAnimals$$014770
000007535 650__ $$aHeart$$019927
000007535 6531_ $$aelectron microscopy
000007535 6531_ $$aanimal models
000007535 6531_ $$acongenital heart defects
000007535 691__ $$aSchool of Medicine$$041369
000007535 692__ $$aDepartment of Biomedical Engineering$$041397
000007535 7001_ $$aRykiel, Graham S.
000007535 8564_ $$9f0eb3ceb-9a84-4fe2-a94e-50f3c9cd77cb$$s4438367$$uhttps://digitalcollections.ohsu.edu/record/7535/files/rykiel.graham.2019.pdf
000007535 905__ $$a/rest/prod/2b/88/qc/73/2b88qc733
000007535 909CO $$ooai:digitalcollections.ohsu.edu:7535$$pstudent-work
000007535 980__ $$aTheses and Dissertations