000000755 001__ 755
000000755 005__ 20260120144838.0
000000755 0247_ $$2DOI$$a10.6083/M4251G61
000000755 037__ $$aETD
000000755 245__ $$aCharacterization of the CD8+T cells following vaccination with an inactivated whole virus vaccine
000000755 260__ $$bOregon Health and Science University
000000755 269__ $$a2011
000000755 336__ $$aDissertation
000000755 502__ $$bPh.D.
000000755 502__ $$gMolecular Microbiology & Immunology (sunsetting)
000000755 520__ $$aLymphocytic choriomeningitis virus (LCMV) infection in mice provides a robust model for studying CD8⁺ T cell immunity. This dissertation demonstrates that vaccination with hydrogen peroxide–inactivated LCMV induces durable, protective CD8⁺ T cell responses with an altered immunodominance hierarchy compared to infection. Vaccine-induced CD8⁺ T cells exhibited enhanced multifunctional cytokine expression and conferred protection against chronic LCMV-Clone 13 challenge, mediated directly by CD8⁺ T cells. Peptide-based studies revealed unprecedented cross-reactivity among LCMV epitopes with minimal sequence homology. These findings establish whole-virus inactivation as a viable vaccine strategy for eliciting strong CD8⁺ T cell immunity and uncover novel cross-reactivity patterns in antiviral responses.
000000755 540__ $$fCC BY
000000755 542__ $$fIn copyright - single owner
000000755 650__ $$aNeuromuscular Junction$$022853
000000755 650__ $$aMyasthenia Gravis$$022558
000000755 650__ $$aSynapses$$026704
000000755 650__ $$aAcetylcholine$$014113
000000755 650__ $$aPhysiology$$024123
000000755 650__ $$aZebrafish$$028076
000000755 650__ $$aPterygium$$024876
000000755 650__ $$aVaccination$$027691
000000755 650__ $$aMyasthenic Syndromes, Congenital$$032298
000000755 650__ $$aReceptors, Cholinergic$$025186
000000755 650__ $$aMice$$036842
000000755 650__ $$aLymphocytic choriomeningitis virus$$021692
000000755 6531_ $$azebra danio
000000755 6531_ $$amyoneural junction
000000755 691__ $$aSchool of Medicine$$041369
000000755 692__ $$aDepartment of Molecular Microbiology and Immunology$$041429
000000755 7001_ $$aWalker, Joshua$$uOregon Health and Science University$$041354
000000755 7201_ $$aSlifka, Mark$$uOregon Health and Science University$$041354$$7Personal$$eAdvisor$$eMentor
000000755 8564_ $$9b9ef537b-0762-4769-97ab-2ff8db3eb45c$$s12632846$$uhttps://digitalcollections.ohsu.edu/record/755/files/758_etd.pdf$$ePublic$$25c537b7809ef815ac453e9b548745585$$31
000000755 905__ $$a/rest/prod/m3/26/m1/75/m326m1757
000000755 909CO $$ooai:digitalcollections.ohsu.edu:755$$pstudent-work
000000755 956__ $$aGet Accessible Copy$$uhttps://ohsu.libwizard.com/f/requestaccessibledocument
000000755 980__ $$aTheses and Dissertations