000007638 001__ 7638
000007638 005__ 20231129124935.0
000007638 0247_ $$2DOI$$a10.6083/m4gm86v3
000007638 037__ $$aETD
000007638 245__ $$aSpatiotemporal dynamics of human epidermal growth factor receptor 2 (her2) protrusion signaling
000007638 260__ $$bOregon Health and Science University
000007638 269__ $$a2017
000007638 336__ $$aDissertation
000007638 502__ $$bPh.D.
000007638 520__ $$aSignal transduction entails the precise interaction of relevant proteins for transmission of signaling pathways that carry out essential functions. In cancer, dysregulated protrusion growth underlies metastasis and disease progression.  Breast cancer cell protrusions express HER2 and HER2 has been linked to protrusion growth, but details of how HER2 signaling is conducted in protrusions remains unclear.  Here, I demonstrate a sensitive and quantitative method for analyzing the expression and spatial localization of HER2 and pAKT using quantum dots (QDs).  I also employ novel monovalent HER2-QD probes using various imaging modalities (single particle tracking, EM, and super-resolution) to characterize the spatiotemporal features of HER2 signaling in breast cancer cell protrusions.
000007638 542__ $$fIn copyright - single owner
000007638 650__ $$aErbB Receptors$$040807
000007638 650__ $$aActins$$014196
000007638 650__ $$aPseudopodia$$024807
000007638 691__ $$aSchool of Medicine$$041369
000007638 692__ $$aDepartment of Biomedical Engineering$$041397
000007638 7001_ $$aLam, Wai Y.
000007638 8564_ $$9a74a45ad-8804-47a7-9e59-dd6fb5b40e43$$s102790917$$uhttps://digitalcollections.ohsu.edu/record/7638/files/Lam.Wai.2017.pdf
000007638 905__ $$a/rest/prod/98/80/vr/59/9880vr597
000007638 909CO $$ooai:digitalcollections.ohsu.edu:7638$$pstudent-work
000007638 980__ $$aTheses and Dissertations