TY  - GEN
N2  - In this dissertation, the rhesus macaque embryo was evaluated for aneuploidy frequency to determine if it is a suitable translation model to study human embryology. Using single-cell  DNA-Sequencing, chromosome  instability  was  established  in  the  rhesus  macaque monkey model and found to occur in 74% of embryos at a nearly identical rate to human embryos. Additional findings of reciprocal sub-chromosomal deletions and duplications support previous reports that chromosome breakage is common in human embryos. Pairing sequencing technology  with  non-invasive  live-cell  time-lapse  imaging  revealed that multipolar divisions were present as well and often resulted in chaotic aneuploidy, which is defined as greater than five whole or segmental chromosomes affected in at least one blastomere where chromosome distribution amongst cells appeared unconstrained and random.
DO  - 10.6083/m4ks6r3r
DO  - DOI
AB  - In this dissertation, the rhesus macaque embryo was evaluated for aneuploidy frequency to determine if it is a suitable translation model to study human embryology. Using single-cell  DNA-Sequencing, chromosome  instability  was  established  in  the  rhesus  macaque monkey model and found to occur in 74% of embryos at a nearly identical rate to human embryos. Additional findings of reciprocal sub-chromosomal deletions and duplications support previous reports that chromosome breakage is common in human embryos. Pairing sequencing technology  with  non-invasive  live-cell  time-lapse  imaging  revealed that multipolar divisions were present as well and often resulted in chaotic aneuploidy, which is defined as greater than five whole or segmental chromosomes affected in at least one blastomere where chromosome distribution amongst cells appeared unconstrained and random.
T1  - Cellular fragmentation-mediated chromosome elimination and blastomere exclusion: mechanisms for overcoming chromosome missegregation in the rhesus macaque preimplantation embryo.
DA  - 2017
AU  - Daughtry, Brittany L.
L1  - https://digitalcollections.ohsu.edu/record/7645/files/Daughtry.Brittany.2017.pdf
PB  - Oregon Health and Science University
PY  - 2017
ID  - 7645
L4  - https://digitalcollections.ohsu.edu/record/7645/files/Daughtry.Brittany.2017.pdf
KW  - Preimplantation Diagnosis
KW  - Cell Nucleus
KW  - Macaca mulatta
KW  - Time-Lapse Imaging
KW  - Aneuploidy
KW  - Single-Cell Analysis
KW  - Chromosomes
TI  - Cellular fragmentation-mediated chromosome elimination and blastomere exclusion: mechanisms for overcoming chromosome missegregation in the rhesus macaque preimplantation embryo.
Y1  - 2017
L2  - https://digitalcollections.ohsu.edu/record/7645/files/Daughtry.Brittany.2017.pdf
LK  - https://digitalcollections.ohsu.edu/record/7645/files/Daughtry.Brittany.2017.pdf
UR  - https://digitalcollections.ohsu.edu/record/7645/files/Daughtry.Brittany.2017.pdf
ER  -