000000766 001__ 766
000000766 005__ 20251216142603.0
000000766 0247_ $$2DOI$$a10.6083/M4MW2F5R
000000766 037__ $$aETD
000000766 245__ $$aGPCR signal attenuation: biochemical and spectroscopic analysis of interaction with affiliate proteins
000000766 260__ $$bOregon Health and Science University
000000766 269__ $$a2012
000000766 336__ $$aDissertation
000000766 502__ $$bPh.D.
000000766 520__ $$aG protein-coupled receptors (GPCRs) mediate essential transmembrane signaling, requiring precise attenuation by regulatory proteins. This work examines interactions between rhodopsin and its modulators, including arrestins and rhodopsin kinase (RK). We show that both visual and β-arrestin 1 preferentially bind monomeric rhodopsin in nanodiscs, and RK phosphorylates monomeric rhodopsin via a conserved binding region shared with transducin. Fluorescence studies identified two arrestin sites interacting with rhodopsin’s TM6 base. Additionally, initial characterization of CRIP1a, a CB1 receptor modulator, revealed high β-sheet content and stability, with ongoing crystallization efforts. These findings advance understanding of GPCR signal termination and structural regulation.
000000766 540__ $$fCC BY
000000766 542__ $$fIn copyright - single owner
000000766 650__ $$aFluorescence$$019121
000000766 650__ $$aG-Protein-Coupled Receptor Kinase 1$$036928
000000766 650__ $$aRhodopsin$$025463
000000766 650__ $$aCarrier Proteins$$016184
000000766 650__ $$aArrestin$$031593
000000766 650__ $$aReceptors, G-Protein-Coupled$$035314
000000766 691__ $$aSchool of Medicine$$041369
000000766 692__ $$aDepartment of Biochemistry and Molecular Biology$$041396
000000766 7001_ $$aSinha, Abhinav$$uOregon Health and Science University$$041354
000000766 7201_ $$aFarrens, David$$uOregon Health and Science University$$041354$$7Personal$$eAdvisor
000000766 8564_ $$93991d089-f017-4b95-a72b-0d5ac47f1fd9$$s6594130$$uhttps://digitalcollections.ohsu.edu/record/766/files/769_etd.pdf$$ePublic$$2cffb169c22968fbc594087a01a26180e$$31
000000766 905__ $$a/rest/prod/gh/93/gz/55/gh93gz55n
000000766 909CO $$ooai:digitalcollections.ohsu.edu:766$$pstudent-work
000000766 980__ $$aTheses and Dissertations