000007660 001__ 7660
000007660 005__ 20240124114248.0
000007660 0247_ $$2DOI$$a10.6083/m4833rk6
000007660 037__ $$aETD
000007660 245__ $$aProbing biological nano-structures with advanced optical techniques
000007660 260__ $$bOregon Health and Science University
000007660 269__ $$a2017
000007660 336__ $$aDissertation
000007660 502__ $$bPh.D.
000007660 520__ $$aThis thesis work focuses on technical developments in the field of superresolution fluorescence microscopy (SRM) and applications of SRM to probing the spatial organization of biological systems at the nanometer and single-molecule scales. Fluorescence microscopy has been a powerful and essential tool for analyzing biological structures and processes due to its noninvasiveness and high molecular specificity. The spatial resolution of conventional fluorescence microscopy, however, is limited to about 250 nm by the diffraction of light. In the last decade or so, a suite of SRM techniques such as stimulated emission depletion (STED) microscopy, photoactivated localization microscopy (PALM) and stochastic optical reconstruction microscopy (STORM), have been invented to circumvent the diffraction limited spatial resolution of fluorescence microscopy. Among these, PALM and STORM are both based on subdiffractive localization of single fluorescent molecules and are the primary technical basis of the present work.
000007660 542__ $$fIn copyright - single owner
000007660 650__ $$aErbB Receptors$$040807
000007660 650__ $$aParaffin Embedding$$029260
000007660 650__ $$aMicroscopy$$022282
000007660 6531_ $$afluorescence microscopy
000007660 691__ $$aSchool of Medicine$$041369
000007660 692__ $$aDepartment of Biomedical Engineering$$041397
000007660 7001_ $$aWang, Jing
000007660 8564_ $$9ef0048b9-1125-4569-a510-d3820975f210$$s8226422$$uhttps://digitalcollections.ohsu.edu/record/7660/files/Wang.Jing.2017.pdf
000007660 905__ $$a/rest/prod/1g/05/fc/30/1g05fc30x
000007660 909CO $$ooai:digitalcollections.ohsu.edu:7660$$pstudent-work
000007660 980__ $$aTheses and Dissertations