TY  - GEN
N2  - Multicolor microscopy tools necessary to localize and visualize the complexity of subcellular systems are limited by current fluorophore technology. While commercial fluorophores cover spectral space from the ultraviolet to the near infrared region and are optimized for conventional bandpass based fluorescence microscopy, they are not ideal for multicolor microscopy as they tend to have short Stokes shifts, restricting the number of fluorophores that can be detected in a single sample to four or five. Herein, we synthesized a library of 95 novel BODIPY (boron-dipyrromethene)-based fluorophores and screened their photophysical properties for conventional and superresolution multicolor microscopy applications. Overall, the fluorophores generated from our BODIPY-based library will enable both conventional and SRM multicolor imaging applications alone or in conjunction with commercially available fluorophores, increasing the available color palette for imaging.
DO  - 10.6083/m4xs5twm
DO  - DOI
AB  - Multicolor microscopy tools necessary to localize and visualize the complexity of subcellular systems are limited by current fluorophore technology. While commercial fluorophores cover spectral space from the ultraviolet to the near infrared region and are optimized for conventional bandpass based fluorescence microscopy, they are not ideal for multicolor microscopy as they tend to have short Stokes shifts, restricting the number of fluorophores that can be detected in a single sample to four or five. Herein, we synthesized a library of 95 novel BODIPY (boron-dipyrromethene)-based fluorophores and screened their photophysical properties for conventional and superresolution multicolor microscopy applications. Overall, the fluorophores generated from our BODIPY-based library will enable both conventional and SRM multicolor imaging applications alone or in conjunction with commercially available fluorophores, increasing the available color palette for imaging.
T1  - Synthesis and characterization of bodipy-based fluorophores for multicolor microscopy
DA  - 2017
AU  - Bittel, Amy M.
L1  - https://digitalcollections.ohsu.edu/record/7681/files/Bittel.Amy.2017.pdf
PB  - Marylhurst University: Oregon Health and Science University
PY  - 2017
ID  - 7681
L4  - https://digitalcollections.ohsu.edu/record/7681/files/Bittel.Amy.2017.pdf
KW  - Boron Compounds
KW  - Single Molecule Imaging
KW  - Microscopy
KW  - Fluorescent Dyes
TI  - Synthesis and characterization of bodipy-based fluorophores for multicolor microscopy
Y1  - 2017
L2  - https://digitalcollections.ohsu.edu/record/7681/files/Bittel.Amy.2017.pdf
LK  - https://digitalcollections.ohsu.edu/record/7681/files/Bittel.Amy.2017.pdf
UR  - https://digitalcollections.ohsu.edu/record/7681/files/Bittel.Amy.2017.pdf
ER  -