000007681 001__ 7681
000007681 005__ 20231129124931.0
000007681 0247_ $$2DOI$$a10.6083/m4xs5twm
000007681 037__ $$aETD
000007681 245__ $$aSynthesis and characterization of bodipy-based fluorophores for multicolor microscopy
000007681 260__ $$bMarylhurst University: Oregon Health and Science University
000007681 269__ $$a2017
000007681 336__ $$aDissertation
000007681 502__ $$bPh.D.
000007681 520__ $$aMulticolor microscopy tools necessary to localize and visualize the complexity of subcellular systems are limited by current fluorophore technology. While commercial fluorophores cover spectral space from the ultraviolet to the near infrared region and are optimized for conventional bandpass based fluorescence microscopy, they are not ideal for multicolor microscopy as they tend to have short Stokes shifts, restricting the number of fluorophores that can be detected in a single sample to four or five. Herein, we synthesized a library of 95 novel BODIPY (boron-dipyrromethene)-based fluorophores and screened their photophysical properties for conventional and superresolution multicolor microscopy applications. Overall, the fluorophores generated from our BODIPY-based library will enable both conventional and SRM multicolor imaging applications alone or in conjunction with commercially available fluorophores, increasing the available color palette for imaging.
000007681 542__ $$fIn copyright - single owner
000007681 650__ $$aBoron Compounds$$015766
000007681 650__ $$aSingle Molecule Imaging$$012091
000007681 650__ $$aMicroscopy$$022282
000007681 650__ $$aFluorescent Dyes$$019124
000007681 691__ $$aSchool of Medicine$$041369
000007681 692__ $$aDepartment of Biomedical Engineering$$041397
000007681 7001_ $$aBittel, Amy M.
000007681 8564_ $$9bb4b63ec-d288-4757-9c5f-2768d4ec024f$$s6452101$$uhttps://digitalcollections.ohsu.edu/record/7681/files/Bittel.Amy.2017.pdf
000007681 905__ $$a/rest/prod/2b/88/qc/75/2b88qc75n
000007681 909CO $$ooai:digitalcollections.ohsu.edu:7681$$pstudent-work
000007681 980__ $$aTheses and Dissertations