000007684 001__ 7684
000007684 005__ 20231129124936.0
000007684 0247_ $$2DOI$$a10.6083/m4zw1kct
000007684 037__ $$aETD
000007684 245__ $$aDissecting functional domains of the resd transcription factor by mutational analysis
000007684 260__ $$bMarylhurst University: Oregon Health and Science University
000007684 269__ $$a2017
000007684 336__ $$aThesis
000007684 502__ $$bM.S.
000007684 520__ $$aRegardless of oxygen availability, Bacillus subtilis is able to survive by maintaining highly regulated and coordinated metabolic pathways, with activation or repression of target genes and/or modulation of protein activity. The ResDE two-component regulatory system of B. subtilis controls gene expression globally at the transcriptional level in order to allow for either aerobic respiration or anaerobic nitrate respiration. Our long-term research goal is to understand how ResD is able to respond to various environmental signals including oxygen and NO and display altered transcriptional regulation depending on the presence of and interactions with RNA polymerase (RNAP) and other transcription factors (TFs). A major aim of this work is to further current understanding of the mechanistic and functional interactions between ResD and another RNAP-associated TF, Spx, under nitrate respiration conditions. B. subtilis relies on the global transcriptional regulator, Spx, to exert both positive and negative control over transcription initiation in response to oxidative stress. ResD and Spx likely coordinate interactions with RNAP in a manner that supports growth under aerobic or anaerobic conditions as the two TFs function under opposite physiological conditions in terms of oxygen levels.
000007684 650__ $$aOxidative Stress $$030758
000007684 650__ $$aTranscription Factors $$027255
000007684 650__ $$aBacillus subtilis $$015316
000007684 650__ $$aTranscriptional Activation$$028481
000007684 650__ $$aOxygen $$023434
000007684 650__ $$aAmino Acid Substitution $$032012
000007684 691__ $$aSchool of Medicine$$041369
000007684 692__ $$aDepartment of Environmental and Biomolecular Systems$$041414
000007684 7001_ $$aJacob, Hannah
000007684 8564_ $$907700cd4-cad7-4b5d-bfb1-e59a91c88698$$s3075139$$uhttps://digitalcollections.ohsu.edu/record/7684/files/Jacob.Hannah.2017.pdf
000007684 905__ $$a/rest/prod/dr/26/xx/89/dr26xx89m
000007684 909CO $$ooai:digitalcollections.ohsu.edu:7684$$pstudent-work
000007684 980__ $$aTheses and Dissertations