000007715 001__ 7715
000007715 005__ 20231129124942.0
000007715 0247_ $$2DOI$$a10.6083/3t945r47j
000007715 037__ $$aETD
000007715 245__ $$aProbing the spatiotemporal dynamics of Ras GTPases on the cell membrane with high-throughput single-molecule microscopy
000007715 260__ $$bMarylhurst University: Oregon Health and Science University
000007715 269__ $$a2019
000007715 336__ $$aDissertation
000007715 502__ $$bPh.D.
000007715 520__ $$aRat sarcoma viral oncogene homolog (Ras) proteins are important signaling molecules ubiquitously expressed in eukaryotes and are key regulators of normal proliferation and differentiation. The Ras family is a group of small GTPases that reside on the membrane and are involved in multiple cell signaling cascades.They transmit growth signals from cell surface receptors, such as tyrosine kinase receptors, by switching between GTP or GDP bound states. Ras is inactive when GDP bound, but stimulation by upstream factors results in the exchange of GDP for GTP with the aid of guanine exchange factors (GEFs). GTP bound Ras is able to bind and recruit downstream electors to the membrane. Although Ras is able to hydrolyze GTP to GDP, the endogenous reaction is slow and is catalyzed by GTPase-activating proteins (GAPs). A third of all human cancers have a constitutively active Ras mutation, making Ras one of the most frequently mutated oncogenes. Oncogenic Ras mutations are single base substitutions that stabilize GTP-bound state which results in constitutive activation of Ras and its target proteins, leading to several hallmarks of cancer.
000007715 542__ $$fIn copyright - single owner
000007715 650__ $$aMicroscopy$$022282
000007715 650__ $$aras Proteins$$030965
000007715 650__ $$aSignal Transduction$$028380
000007715 650__ $$aDiffusion$$017802
000007715 691__ $$aSchool of Medicine$$041369
000007715 7001_ $$aLee, Yerim
000007715 8564_ $$9661c1f0a-c5ca-41b8-9296-3860d2828113$$s9877157$$uhttps://digitalcollections.ohsu.edu/record/7715/files/lee.yerim.2019.pdf
000007715 905__ $$a/rest/prod/3t/94/5r/47/3t945r47j
000007715 909CO $$ooai:digitalcollections.ohsu.edu:7715$$pstudent-work
000007715 980__ $$aTheses and Dissertations