000000799 001__ 799
000000799 005__ 20260420142755.0
000000799 0247_ $$2DOI$$a10.6083/M4C82787
000000799 037__ $$aETD
000000799 245__ $$aDevelopment of novel human reporter cell lines using recombinant adeno-associated virus mediated homologous recombination
000000799 260__ $$bOregon Health and Science University
000000799 269__ $$a2007
000000799 336__ $$aThesis
000000799 502__ $$bM.S.
000000799 520__ $$aUnderstanding gene regulation is critical for developing novel therapeutic strategies. This study presents a new method for generating human cell lines that function as reporters of transcriptional activity using recombinant adeno-associated virus (rAAV)–mediated homologous recombination. To overcome rAAV cargo size limitations, an EGFP–luciferase fusion gene was employed as both a selectable marker and transcriptional reporter. EGFP-based selection leveraged endogenous gene activation conditions to identify correctly targeted alleles. Using this approach, primary human fibroblast lines were generated in which EGFP–luciferase expression is driven by the endogenous c-Myc oncogene, providing a powerful tool for studying transcriptional regulation in human cells.
000000799 540__ $$fCC BY
000000799 542__ $$fIn copyright - single owner
000000799 650__ $$aFibroblasts$$019020
000000799 650__ $$aOncogenes$$023203
000000799 650__ $$aGenes$$019447
000000799 650__ $$aCell Line$$016284
000000799 650__ $$aGene Expression Regulation$$019437
000000799 650__ $$aGene Targeting$$030764
000000799 650__ $$aHomologous Recombination$$039630
000000799 691__ $$aSchool of Medicine$$041369
000000799 692__ $$aDepartment of Cell, Developmental and Cancer Biology$$041399
000000799 7001_ $$aFernandez, Sandra$$uOregon Health and Science University$$041354
000000799 8564_ $$9f14331fc-1e8b-4298-aed8-afd783ae0126$$s3417101$$uhttps://digitalcollections.ohsu.edu/record/799/files/802_etd.pdf$$ePublic$$25616337c1519dcc93540b9ac571e0200$$31
000000799 901__ $$a&lt;p&gt;These documents are archival records. They are retained for historical reference only. &lt;/p&gt;&lt;p&gt;&lt;b&gt;Need an accessible version? Use the ‘Get Accessible Copy’ link above.&lt;/b&gt;&lt;/p&gt;
000000799 905__ $$a/rest/prod/nc/58/0m/70/nc580m70f
000000799 909CO $$ooai:digitalcollections.ohsu.edu:799$$pstudent-work
000000799 956__ $$aGet Accessible Copy$$uhttps://ohsu.libwizard.com/f/requestaccessibledocument
000000799 980__ $$aTheses and Dissertations