TY  - GEN
N2  - Biological imaging relies on the ability to generate contrast between the target of interest and the cell. Proteins are specifically labeled with reporters compatible with either light microscopy or electron microscopy or both. This chapter provides a broad overview of methods for protein labeling for imaging and a description of Versatile Interacting Peptide (VIP) tags, a new technology for labeling proteins for microscopy. VIP tags are discussed herein in the context of: 1) peptide tags for imaging and 2) tags for electron microscopy. VIP tags are part of an effort to develop small peptide tags (<7 kDa), without sacrificing specificity or utility. VIP tags are also a new genetically encoded tag for electron microscopy, which has far fewer options compared to protein tags for fluorescent imaging.
DO  - 10.6083/0g354f922
DO  - DOI
AB  - Biological imaging relies on the ability to generate contrast between the target of interest and the cell. Proteins are specifically labeled with reporters compatible with either light microscopy or electron microscopy or both. This chapter provides a broad overview of methods for protein labeling for imaging and a description of Versatile Interacting Peptide (VIP) tags, a new technology for labeling proteins for microscopy. VIP tags are discussed herein in the context of: 1) peptide tags for imaging and 2) tags for electron microscopy. VIP tags are part of an effort to develop small peptide tags (<7 kDa), without sacrificing specificity or utility. VIP tags are also a new genetically encoded tag for electron microscopy, which has far fewer options compared to protein tags for fluorescent imaging.
T1  - The development and application of versatile interacting peptide (VIP) tags for advanced imaging
DA  - 2020
AU  - Doh, Julia K.
L1  - https://digitalcollections.ohsu.edu/record/8081/files/Doh.Julia.2020.pdf
PB  - Oregon Health and Science University
PY  - 2020
ID  - 8081
L4  - https://digitalcollections.ohsu.edu/record/8081/files/Doh.Julia.2020.pdf
KW  - Iron
KW  - Peptides
KW  - fluorescence microscopy
KW  - electron microscopy
KW  - chemical biology
TI  - The development and application of versatile interacting peptide (VIP) tags for advanced imaging
Y1  - 2020
L2  - https://digitalcollections.ohsu.edu/record/8081/files/Doh.Julia.2020.pdf
LK  - https://digitalcollections.ohsu.edu/record/8081/files/Doh.Julia.2020.pdf
UR  - https://digitalcollections.ohsu.edu/record/8081/files/Doh.Julia.2020.pdf
ER  -