000008081 001__ 8081
000008081 005__ 20240124114258.0
000008081 0247_ $$2DOI$$a10.6083/0g354f922
000008081 037__ $$aETD
000008081 245__ $$aThe development and application of versatile interacting peptide (VIP) tags for advanced imaging
000008081 260__ $$bOregon Health and Science University
000008081 269__ $$a2020
000008081 336__ $$aDissertation
000008081 502__ $$bPh.D.
000008081 520__ $$aBiological imaging relies on the ability to generate contrast between the target of interest and the cell. Proteins are specifically labeled with reporters compatible with either light microscopy or electron microscopy or both. This chapter provides a broad overview of methods for protein labeling for imaging and a description of Versatile Interacting Peptide (VIP) tags, a new technology for labeling proteins for microscopy. VIP tags are discussed herein in the context of: 1) peptide tags for imaging and 2) tags for electron microscopy. VIP tags are part of an effort to develop small peptide tags (<7 kDa), without sacrificing specificity or utility. VIP tags are also a new genetically encoded tag for electron microscopy, which has far fewer options compared to protein tags for fluorescent imaging.
000008081 542__ $$fIn copyright - single owner
000008081 650__ $$aIron$$021036
000008081 650__ $$aPeptides$$023769
000008081 6531_ $$afluorescence microscopy
000008081 6531_ $$aelectron microscopy
000008081 6531_ $$achemical biology
000008081 691__ $$aSchool of Medicine$$041369
000008081 7001_ $$aDoh, Julia K.
000008081 8564_ $$928b69ce9-2ae0-4050-ac3d-0af3e5d4d587$$s11547619$$uhttps://digitalcollections.ohsu.edu/record/8081/files/Doh.Julia.2020.pdf
000008081 905__ $$a/rest/prod/0g/35/4f/92/0g354f922
000008081 909CO $$ooai:digitalcollections.ohsu.edu:8081$$pstudent-work
000008081 980__ $$aTheses and Dissertations