000000809 001__ 809
000000809 005__ 20260423132719.0
000000809 0247_ $$2DOI$$a10.6083/M42V2D4K
000000809 037__ $$aETD
000000809 245__ $$aThe role of the human excision repair cross complementation (group 1) protein in the fanconi anemia pathway
000000809 260__ $$bOregon Health and Science University
000000809 269__ $$a2007
000000809 336__ $$aDissertation
000000809 502__ $$bPh.D.
000000809 502__ $$gMolecular & Medical Genetics (sunsetting)
000000809 520__ $$aThe Fanconi anemia (FA) pathway preserves genomic stability by coordinating responses to DNA interstrand crosslinks (ICLs) and replication stress. This study examines the role of the ERCC1/XPF endonuclease in FA pathway activation. Using ERCC1-depleted human fibroblasts, we show that ERCC1 is not required for double-strand break formation following exposure to MMC, DEB, or HU, but is essential for FANCD2 monoubiquitination. ERCC1 depletion also reduces radial chromosome formation when combined with FANCA deficiency. These findings place ERCC1 downstream of H2AX phosphorylation and upstream of FA pathway activation, suggesting the FA pathway responds to replication-associated intermediates rather than double-strand breaks.
000000809 540__ $$fCC BY
000000809 542__ $$fIn copyright - single owner
000000809 650__ $$aDNA Repair$$017990
000000809 650__ $$aFanconi Anemia$$018886
000000809 650__ $$aHydroxyurea$$020483
000000809 650__ $$aEpoxy Compounds$$018555
000000809 650__ $$aMitomycin$$029322
000000809 650__ $$aGenomic Instability$$035232
000000809 691__ $$aSchool of Medicine$$041369
000000809 692__ $$aDepartment of Molecular and Medical Genetics$$041428
000000809 7001_ $$aMcCabe, Kevin$$uOregon Health and Science University$$041354
000000809 8564_ $$9f79cd0f8-ec4d-4d32-8f0d-4c9f2c36b992$$s1461371$$uhttps://digitalcollections.ohsu.edu/record/809/files/812_etd.pdf$$ePublic$$2ca3d287bf7fb760aff2b03e51566fce1$$31
000000809 901__ $$a<p>These documents are archival records. They are retained for historical reference only. </p><p><b>Need an accessible version? Use the ‘Get Accessible Copy’ link above.</b></p>
000000809 905__ $$a/rest/prod/05/74/1r/72/05741r72k
000000809 909CO $$ooai:digitalcollections.ohsu.edu:809$$pstudent-work
000000809 956__ $$aGet Accessible Copy$$uhttps://ohsu.libwizard.com/f/requestaccessibledocument
000000809 980__ $$aTheses and Dissertations