@article{IR,
      author = {Shangguan, Julia and Civitci, Fehmi and Tao, Kai and Wang,  Jing and Kwon, Sunjong and Zheng, Ting and Kenison, John  and Rames, Matthew and Nan, Xiaolin},
      url = {http://digitalcollections.ohsu.edu/record/8252},
      title = {Fast, multiplexed superresolution imaging of HER2  signaling in breast cancer with DNA-PAINT-ERS},
      publisher = {Oregon Health and Science University},
      abstract = {Super resolution microscopy (SRM) comprises various  single-molecule localization techniques that can generate  images at the 20 nm scale. In recent years, SRM based on  DNA point accumulation in nanoscale topology (DNA-PAINT)  has become increasingly useful for biological imaging for  its robust capability for multiplexing. However, the  practical use of DNA-PAINT has been limited by slow imaging  speed. Here, we introduce DNA-PAINT-ERS, a set of  strategies that can be easily integrated into current  workflows for both accelerated DNA-PAINT and improved image  quality. These advances have allowed us to use  DNA-PAINT-ERS for the imaging of HER2 signaling in breast  cancer. HER2 is a member of the epidermal growth factor  (EGF) receptor family, and HER2 gene amplification and/or  protein over-expression is commonly associated with human  malignancies such as breast cancer. Using multicolor SRM  based on DNA-PAINT-ERS, we can now image many different  targets involved in the nanoscopic organization and  signaling of HER2. The imaging results start to suggest a  new mechanism that could lead to persistent HER2 signaling  upon HER2-targeted therapy, thus contributing to  therapeutic resistance.},
      number = {IR},
      doi = {https://doi.org/10.6083/m900nv06b},
      recid = {8252},
      address = {2020},
}