TY  - GEN
N2  - Super resolution microscopy (SRM) comprises various single-molecule localization techniques that can generate images at the 20 nm scale. In recent years, SRM based on DNA point accumulation in nanoscale topology (DNA-PAINT) has become increasingly useful for biological imaging for its robust capability for multiplexing. However, the practical use of DNA-PAINT has been limited by slow imaging speed. Here, we introduce DNA-PAINT-ERS, a set of strategies that can be easily integrated into current workflows for both accelerated DNA-PAINT and improved image quality. These advances have allowed us to use DNA-PAINT-ERS for the imaging of HER2 signaling in breast cancer. HER2 is a member of the epidermal growth factor (EGF) receptor family, and HER2 gene amplification and/or protein over-expression is commonly associated with human malignancies such as breast cancer. Using multicolor SRM based on DNA-PAINT-ERS, we can now image many different targets involved in the nanoscopic organization and signaling of HER2. The imaging results start to suggest a new mechanism that could lead to persistent HER2 signaling upon HER2-targeted therapy, thus contributing to therapeutic resistance.
DO  - 10.6083/m900nv06b
DO  - DOI
AB  - Super resolution microscopy (SRM) comprises various single-molecule localization techniques that can generate images at the 20 nm scale. In recent years, SRM based on DNA point accumulation in nanoscale topology (DNA-PAINT) has become increasingly useful for biological imaging for its robust capability for multiplexing. However, the practical use of DNA-PAINT has been limited by slow imaging speed. Here, we introduce DNA-PAINT-ERS, a set of strategies that can be easily integrated into current workflows for both accelerated DNA-PAINT and improved image quality. These advances have allowed us to use DNA-PAINT-ERS for the imaging of HER2 signaling in breast cancer. HER2 is a member of the epidermal growth factor (EGF) receptor family, and HER2 gene amplification and/or protein over-expression is commonly associated with human malignancies such as breast cancer. Using multicolor SRM based on DNA-PAINT-ERS, we can now image many different targets involved in the nanoscopic organization and signaling of HER2. The imaging results start to suggest a new mechanism that could lead to persistent HER2 signaling upon HER2-targeted therapy, thus contributing to therapeutic resistance.
AD  - Oregon Health and Science University
AD  - Oregon Health and Science University
AD  - Oregon Health and Science University
AD  - Oregon Health and Science University
AD  - Oregon Health and Science University
AD  - Oregon Health and Science University
AD  - Oregon Health and Science University
AD  - Oregon Health and Science University
AD  - Oregon Health and Science University
T1  - Fast, multiplexed superresolution imaging of HER2 signaling in breast cancer with DNA-PAINT-ERS
DA  - 2020
AU  - Shangguan, Julia
AU  - Civitci, Fehmi
AU  - Tao, Kai
AU  - Wang, Jing
AU  - Kwon, Sunjong
AU  - Zheng, Ting
AU  - Kenison, John
AU  - Rames, Matthew
AU  - Nan, Xiaolin
L1  - https://digitalcollections.ohsu.edu/record/8252/files/Julia-Shangguan.pdf
PB  - Oregon Health and Science University
LA  - eng
PY  - 2020
ID  - 8252
L4  - https://digitalcollections.ohsu.edu/record/8252/files/Julia-Shangguan.pdf
KW  - Microscopy
KW  - Receptor, ErbB-2
KW  - Breast Neoplasms
KW  - her2
KW  - breast cancer
KW  - dna-paint-ers
KW  - super resolution microscopy
KW  - cancer
TI  - Fast, multiplexed superresolution imaging of HER2 signaling in breast cancer with DNA-PAINT-ERS
Y1  - 2020
L2  - https://digitalcollections.ohsu.edu/record/8252/files/Julia-Shangguan.pdf
LK  - https://digitalcollections.ohsu.edu/record/8252/files/Julia-Shangguan.pdf
UR  - https://digitalcollections.ohsu.edu/record/8252/files/Julia-Shangguan.pdf
ER  -