000008252 001__ 8252
000008252 005__ 20240405155343.0
000008252 0247_ $$2DOI$$a10.6083/m900nv06b
000008252 037__ $$aIR
000008252 041__ $$aeng
000008252 245__ $$aFast, multiplexed superresolution imaging of HER2 signaling in breast cancer with DNA-PAINT-ERS
000008252 260__ $$bOregon Health and Science University
000008252 269__ $$a2020
000008252 336__ $$aAbstract
000008252 520__ $$aSuper resolution microscopy (SRM) comprises various single-molecule localization techniques that can generate images at the 20 nm scale. In recent years, SRM based on DNA point accumulation in nanoscale topology (DNA-PAINT) has become increasingly useful for biological imaging for its robust capability for multiplexing. However, the practical use of DNA-PAINT has been limited by slow imaging speed. Here, we introduce DNA-PAINT-ERS, a set of strategies that can be easily integrated into current workflows for both accelerated DNA-PAINT and improved image quality. These advances have allowed us to use DNA-PAINT-ERS for the imaging of HER2 signaling in breast cancer. HER2 is a member of the epidermal growth factor (EGF) receptor family, and HER2 gene amplification and/or protein over-expression is commonly associated with human malignancies such as breast cancer. Using multicolor SRM based on DNA-PAINT-ERS, we can now image many different targets involved in the nanoscopic organization and signaling of HER2. The imaging results start to suggest a new mechanism that could lead to persistent HER2 signaling upon HER2-targeted therapy, thus contributing to therapeutic resistance.
000008252 540__ $$fCC BY
000008252 542__ $$fIn copyright - joint owners
000008252 650__ $$aMicroscopy$$022282
000008252 650__ $$aReceptor, ErbB-2$$031038
000008252 650__ $$aBreast Neoplasms$$015809
000008252 6531_ $$aher2
000008252 6531_ $$abreast cancer
000008252 6531_ $$adna-paint-ers
000008252 6531_ $$asuper resolution microscopy
000008252 6531_ $$acancer
000008252 691__ $$aSchool of Medicine$$041369
000008252 692__ $$aDepartment of Biomedical Engineering$$041397
000008252 7001_ $$aShangguan, Julia$$uOregon Health and Science University$$041354
000008252 7001_ $$aCivitci, Fehmi$$uOregon Health and Science University$$041354
000008252 7001_ $$aTao, Kai$$uOregon Health and Science University$$041354
000008252 7001_ $$aWang, Jing$$uOregon Health and Science University$$041354
000008252 7001_ $$aKwon, Sunjong$$uOregon Health and Science University$$041354
000008252 7001_ $$aZheng, Ting$$uOregon Health and Science University$$041354
000008252 7001_ $$aKenison, John$$uOregon Health and Science University$$041354
000008252 7001_ $$aRames, Matthew$$uOregon Health and Science University$$041354
000008252 7001_ $$aNan, Xiaolin$$uOregon Health and Science University$$041354
000008252 711__ $$aResearch Week$$uOregon Health and Science University$$d2020
000008252 8564_ $$951e15d1c-8583-4d7d-b79b-85b31f8471b0$$s49286$$uhttps://digitalcollections.ohsu.edu/record/8252/files/Julia-Shangguan.pdf
000008252 905__ $$a/rest/prod/m9/00/nv/06/m900nv06b
000008252 980__ $$aResearch Week