000008260 001__ 8260
000008260 005__ 20240305133547.0
000008260 0247_ $$2DOI$$a10.6083/df65v8687
000008260 037__ $$aIR
000008260 041__ $$aeng
000008260 245__ $$aA novel approach for melanoma circulating tumor cell isolation from patient whole blood
000008260 260__ $$bOregon Health and Science University
000008260 269__ $$a2020
000008260 336__ $$aAbstract
000008260 520__ $$aThe current standard for investigating melanoma, solid tumor biopsy, is a costly and inefficient means of extracting information about disease progression through repeat tumor biopsies. Alternatively, circulating tumor cells (CTCs, cells that have broken away from the primary tumor or metastatic cites) can be extracted from the blood in a liquid biopsy using a minimally invasive blood draw. We hypothesize that analyzing these CTCs both genetically and epigenetically may divulge important insights into melanoma progression, evolution, and response to treatment. The purpose of this study is to develop improved methods for CTC isolation. Here, we demonstrate a novel workflow for isolating melanoma CTCs. We successfully validate this approach by isolating and sequence verifying single A375 melanoma cells enriched from whole blood. Previous strategies for CTC isolation have been problematic; microfluidic approaches to CTC isolation may miss CTCs of aberrant morphology, while other antibody-based CTC isolation strategies are limited by using only a small number of antibodies to label their cells. Our approach does not rely upon cell morphology, and takes advantage of a large cocktail of antibodies tailored specifically for melanoma CTCs to overcome these limitations. Future studies include single cell RNA sequencing to make mechanistic insights into melanoma evolution over the course of immune checkpoint blockade (ICB) therapy targeting the PD-1 axis.
000008260 540__ $$fCC BY
000008260 542__ $$fIn copyright - joint owners
000008260 650__ $$aNeoplastic Cells, Circulating$$022749
000008260 650__ $$aMelanoma$$021989
000008260 650__ $$aSkin Neoplasms$$026054
000008260 650__ $$aImmunotherapy$$020724
000008260 6531_ $$atranscriptomics
000008260 691__ $$aSchool of Medicine$$041369
000008260 692__ $$aDepartment of Dermatology$$041406
000008260 7001_ $$aFankhauser, Reilly$$uOregon Health and Science University$$041354
000008260 7001_ $$aSeifert, Hilary$$uOregon Health and Science University$$041354
000008260 7001_ $$aFuiten, Allison$$uOregon Health and Science University$$041354
000008260 7001_ $$aDePatie, Nick$$uOregon Health and Science University$$041354
000008260 7001_ $$aKulkarni, Rajan$$uOregon Health and Science University$$041354
000008260 711__ $$aResearch Week$$uOregon Health and Science University$$d2020
000008260 8564_ $$9a6bc29ac-d614-4e7d-878d-58deeacbe775$$s45455$$uhttps://digitalcollections.ohsu.edu/record/8260/files/Reilly-Fankhauser.pdf
000008260 905__ $$a/rest/prod/df/65/v8/68/df65v8687
000008260 980__ $$aResearch Week