TY  - GEN
N2  - Intercellular communication by the gap junctions is facilitated by a unique macromolecular architecture, in which transmembrane channels directly couple the plasma membranes of neighboring cells. In each membrane, six connexin (Cx) subunits oligomerize to form a hemichannel. Docking between extracellular (EC) domains of two hemichannels forms the complete gap junction intercellular channel. Our current understanding of gap junction structure lacks crucial information about how these channels interact with their native phospholipid environment. To address this gap in knowledge, we used lipid nanodisc technology to incorporate native connexin-46/50 (Cx46/50) intercellular channels into a dual-lipid bilayer closely mimicking a native cell-to-cell junction.
DO  - 10.6083/3j3332855
DO  - DOI
AB  - Intercellular communication by the gap junctions is facilitated by a unique macromolecular architecture, in which transmembrane channels directly couple the plasma membranes of neighboring cells. In each membrane, six connexin (Cx) subunits oligomerize to form a hemichannel. Docking between extracellular (EC) domains of two hemichannels forms the complete gap junction intercellular channel. Our current understanding of gap junction structure lacks crucial information about how these channels interact with their native phospholipid environment. To address this gap in knowledge, we used lipid nanodisc technology to incorporate native connexin-46/50 (Cx46/50) intercellular channels into a dual-lipid bilayer closely mimicking a native cell-to-cell junction.
AD  - Oregon Health and Science University
AD  - Oregon Health and Science University
AD  - Oregon Health and Science University
AD  - Oregon Health and Science University
AD  - Oregon Health and Science University
AD  - Oregon Health and Science University
AD  - Oregon Health and Science University
T1  - Visualization of intercellular communication channels in a dual-lipid bilayer at near-atomic resolution by cryoEM
DA  - 2020
AU  - Flores, Jonathan
AU  - Haddad, Bassam H.
AU  - Dolan, Kimberly
AU  - Myers, Janette B.
AU  - Yoshioka, Craig C.
AU  - Zuckerman, Daniel M.
AU  - Reichow, Steve L.
L1  - https://digitalcollections.ohsu.edu/record/8317/files/ResearchWeek.2020.Flores.Jonathan.pdf
PB  - Oregon Health and Science University
PY  - 2020
ID  - 8317
L4  - https://digitalcollections.ohsu.edu/record/8317/files/ResearchWeek.2020.Flores.Jonathan.pdf
KW  - Lipids
KW  - Gap Junctions
KW  - Cryoelectron Microscopy
KW  - cryoem
KW  - nanodisc
KW  - hemichannel
KW  - intercellular junctions
KW  - connexin-46
KW  - connexin-50
KW  - cx46/50
KW  - lipid bilayer
TI  - Visualization of intercellular communication channels in a dual-lipid bilayer at near-atomic resolution by cryoEM
Y1  - 2020
L2  - https://digitalcollections.ohsu.edu/record/8317/files/ResearchWeek.2020.Flores.Jonathan.pdf
LK  - https://digitalcollections.ohsu.edu/record/8317/files/ResearchWeek.2020.Flores.Jonathan.pdf
UR  - https://digitalcollections.ohsu.edu/record/8317/files/ResearchWeek.2020.Flores.Jonathan.pdf
ER  -