000008317 001__ 8317
000008317 005__ 20240125142354.0
000008317 0247_ $$2DOI$$a10.6083/3j3332855
000008317 037__ $$aIR
000008317 245__ $$aVisualization of intercellular communication channels in a dual-lipid bilayer at near-atomic resolution by cryoEM
000008317 260__ $$bOregon Health and Science University
000008317 269__ $$a2020
000008317 336__ $$aAbstract
000008317 520__ $$aIntercellular communication by the gap junctions is facilitated by a unique macromolecular architecture, in which transmembrane channels directly couple the plasma membranes of neighboring cells. In each membrane, six connexin (Cx) subunits oligomerize to form a hemichannel. Docking between extracellular (EC) domains of two hemichannels forms the complete gap junction intercellular channel. Our current understanding of gap junction structure lacks crucial information about how these channels interact with their native phospholipid environment. To address this gap in knowledge, we used lipid nanodisc technology to incorporate native connexin-46/50 (Cx46/50) intercellular channels into a dual-lipid bilayer closely mimicking a native cell-to-cell junction.
000008317 540__ $$fCC BY
000008317 542__ $$fIn copyright - joint owners
000008317 650__ $$aLipids
000008317 650__ $$aGap Junctions$$030121
000008317 650__ $$aCryoelectron Microscopy$$032293
000008317 6531_ $$acryoem
000008317 6531_ $$ananodisc
000008317 6531_ $$ahemichannel
000008317 6531_ $$aintercellular junctions
000008317 6531_ $$aconnexin-46
000008317 6531_ $$aconnexin-50
000008317 6531_ $$acx46/50
000008317 6531_ $$alipid bilayer
000008317 691__ $$aSchool of Medicine$$041369
000008317 692__ $$aDepartment of Chemical Physiology and Biochemistry$$041400
000008317 7001_ $$aFlores, Jonathan$$uOregon Health and Science University$$041354
000008317 7001_ $$aHaddad, Bassam H.$$uOregon Health and Science University$$041354
000008317 7001_ $$aDolan, Kimberly$$uOregon Health and Science University$$041354
000008317 7001_ $$aMyers, Janette B.$$uOregon Health and Science University$$041354
000008317 7001_ $$aYoshioka, Craig C.$$uOregon Health and Science University$$041354
000008317 7001_ $$aZuckerman, Daniel M.$$uOregon Health and Science University$$041354
000008317 7001_ $$aReichow, Steve L.$$uOregon Health and Science University$$041354
000008317 711__ $$aResearch Week$$uOregon Health and Science University$$d2020
000008317 8564_ $$9a546d94b-f851-4ab4-9c17-155f1a6bf48f$$s48311$$uhttps://digitalcollections.ohsu.edu/record/8317/files/ResearchWeek.2020.Flores.Jonathan.pdf
000008317 905__ $$a/rest/prod/3j/33/32/85/3j3332855
000008317 980__ $$aResearch Week