TY  - GEN
N2  - Culture-independent methods are changing the way scientists interrogate microbes in complex environments such as the human body. Specifically, targeted sequencing of the conserved 16S rRNA gene is relatively simple, inexpensive, and high-throughput. However, the analysis of such data quantifies each microbe as a fraction of the total read count, which can lead to misinterpretations of microbes that differ between communities associated with disease. To address this limitation, quantitative microbiome profiling (QMP) methods have been developed.
DO  - 10.6083/8049g5613
DO  - DOI
AB  - Culture-independent methods are changing the way scientists interrogate microbes in complex environments such as the human body. Specifically, targeted sequencing of the conserved 16S rRNA gene is relatively simple, inexpensive, and high-throughput. However, the analysis of such data quantifies each microbe as a fraction of the total read count, which can lead to misinterpretations of microbes that differ between communities associated with disease. To address this limitation, quantitative microbiome profiling (QMP) methods have been developed.
AD  - Oregon Health and Science University
AD  - Oregon Health and Science University
AD  - Oregon Health and Science University
AD  - Oregon Health and Science University
AD  - Oregon Health and Science University
AD  - Oregon Health and Science University
AD  - Oregon Health and Science University
T1  - To flow or not to flow: considerations for quantitative microbiome profiling
DA  - 2020
AU  - Bowie, Kathleen
AU  - Furst, Alec
AU  - Schleisman, Matt
AU  - Jallow, Fatoumata
AU  - Davin, Sean
AU  - Rosenbaum, James T.
AU  - Karstens, Lisa
L1  - https://digitalcollections.ohsu.edu/record/8360/files/ResearchWeek.2020.Bowie.Kathleen.pdf
PB  - Oregon Health and Science University
PY  - 2020
ID  - 8360
L4  - https://digitalcollections.ohsu.edu/record/8360/files/ResearchWeek.2020.Bowie.Kathleen.pdf
KW  - quantitative microbiome profiling
KW  - microbial load
KW  - 16S rRNA gene
KW  - microbiome
KW  - gene sequencing
TI  - To flow or not to flow: considerations for quantitative microbiome profiling
Y1  - 2020
L2  - https://digitalcollections.ohsu.edu/record/8360/files/ResearchWeek.2020.Bowie.Kathleen.pdf
LK  - https://digitalcollections.ohsu.edu/record/8360/files/ResearchWeek.2020.Bowie.Kathleen.pdf
UR  - https://digitalcollections.ohsu.edu/record/8360/files/ResearchWeek.2020.Bowie.Kathleen.pdf
ER  -