TY  - GEN
N2  - The use of synthetic mRNA-based gene modification offers several advantages over traditional DNA based gene therapy including faster translational kinetics, transient expression and mitigation of risks associated with insertational mutagenesis and genomic integration. Accordingly, mRNA technology holds particular promise for applications in tissue engineering where spatiotemporal control of gene expression is crucial. Recent advances in non-viral delivery technologies have significantly increased the efficiency of intracellular delivery of synthetic mRNA by modulating mTOR signaling, which has been identified as a crucial player in mRNA translation. However, mTOR signaling is regulated by matrix stiffness through mechanotransductive pathways. Therefore, the mechanical properties of the engineered tissue must be tailored for optimal translation and expression of mRNA.
DO  - 10.6083/jd472x06b
DO  - DOI
AB  - The use of synthetic mRNA-based gene modification offers several advantages over traditional DNA based gene therapy including faster translational kinetics, transient expression and mitigation of risks associated with insertational mutagenesis and genomic integration. Accordingly, mRNA technology holds particular promise for applications in tissue engineering where spatiotemporal control of gene expression is crucial. Recent advances in non-viral delivery technologies have significantly increased the efficiency of intracellular delivery of synthetic mRNA by modulating mTOR signaling, which has been identified as a crucial player in mRNA translation. However, mTOR signaling is regulated by matrix stiffness through mechanotransductive pathways. Therefore, the mechanical properties of the engineered tissue must be tailored for optimal translation and expression of mRNA.
AD  - Oregon Health and Science University
AD  - Oregon Health and Science University
AD  - Oregon Health and Science University
AD  - Oregon Health and Science University
AD  - Oregon Health and Science University
T1  - Role of cellular microenvironment in intracellular mRNA delivery for applications in tissue engineering
DA  - 2020
AU  - Athirasala, Avathamsa
AU  - Patel, Siddharth
AU  - dos Passos Menezes, Paula
AU  - Sahay, Gaurav
AU  - Bertassoni, Luiz E.
L1  - https://digitalcollections.ohsu.edu/record/8400/files/ResearchWeek.2020.Athirasala.Avathamsa.pdf
PB  - Oregon Health and Science University
LA  - eng
PY  - 2020
ID  - 8400
L4  - https://digitalcollections.ohsu.edu/record/8400/files/ResearchWeek.2020.Athirasala.Avathamsa.pdf
KW  - Tissue Engineering
KW  - Mechanotransduction, Cellular
KW  - RNA, Messenger
KW  - Stem Cells
KW  - Signal Transduction
KW  - mRNA
KW  - mTOR signaling
KW  - mTOR
KW  - sensory transduction
TI  - Role of cellular microenvironment in intracellular mRNA delivery for applications in tissue engineering
Y1  - 2020
L2  - https://digitalcollections.ohsu.edu/record/8400/files/ResearchWeek.2020.Athirasala.Avathamsa.pdf
LK  - https://digitalcollections.ohsu.edu/record/8400/files/ResearchWeek.2020.Athirasala.Avathamsa.pdf
UR  - https://digitalcollections.ohsu.edu/record/8400/files/ResearchWeek.2020.Athirasala.Avathamsa.pdf
ER  -