000008400 001__ 8400
000008400 005__ 20240415153538.0
000008400 0247_ $$2DOI$$a10.6083/jd472x06b
000008400 037__ $$aIR
000008400 041__ $$aeng
000008400 245__ $$aRole of cellular microenvironment in intracellular mRNA delivery for applications in tissue engineering
000008400 260__ $$bOregon Health and Science University
000008400 269__ $$a2020
000008400 336__ $$aAbstract
000008400 520__ $$aThe use of synthetic mRNA-based gene modification offers several advantages over traditional DNA based gene therapy including faster translational kinetics, transient expression and mitigation of risks associated with insertational mutagenesis and genomic integration. Accordingly, mRNA technology holds particular promise for applications in tissue engineering where spatiotemporal control of gene expression is crucial. Recent advances in non-viral delivery technologies have significantly increased the efficiency of intracellular delivery of synthetic mRNA by modulating mTOR signaling, which has been identified as a crucial player in mRNA translation. However, mTOR signaling is regulated by matrix stiffness through mechanotransductive pathways. Therefore, the mechanical properties of the engineered tissue must be tailored for optimal translation and expression of mRNA.
000008400 540__ $$fCC BY
000008400 542__ $$fIn copyright - joint owners
000008400 650__ $$aTissue Engineering$$033051
000008400 650__ $$aMechanotransduction, Cellular$$035047
000008400 650__ $$aRNA, Messenger$$025546
000008400 650__ $$aStem Cells$$026391
000008400 650__ $$aSignal Transduction$$028380
000008400 6531_ $$amRNA
000008400 6531_ $$amTOR signaling
000008400 6531_ $$amTOR
000008400 6531_ $$asensory transduction
000008400 691__ $$aSchool of Dentistry$$041368
000008400 692__ $$aDepartment of Restorative Dentistry$$041446
000008400 7001_ $$aAthirasala, Avathamsa$$uOregon Health and Science University$$041354
000008400 7001_ $$aPatel, Siddharth$$uOregon Health and Science University$$041354
000008400 7001_ $$ados Passos Menezes, Paula$$uOregon Health and Science University$$041354
000008400 7001_ $$aSahay, Gaurav$$uOregon Health and Science University$$041354
000008400 7001_ $$aBertassoni, Luiz E.$$uOregon Health and Science University$$041354
000008400 711__ $$aResearch Week$$uOregon Health and Science University$$d2020
000008400 8564_ $$918c44b4e-11e3-428a-9f04-c47506d6692c$$s47993$$uhttps://digitalcollections.ohsu.edu/record/8400/files/ResearchWeek.2020.Athirasala.Avathamsa.pdf
000008400 905__ $$a/rest/prod/jd/47/2x/06/jd472x06b
000008400 980__ $$aResearch Week