TY  - GEN
N2  - In recent studies, c-Myc has been shown to localize to the nuclear pore complex (NPC) after specific post-translational modifications. The localization of c-Myc to the NPC can mediate aspects of stress response to environmental signals, and separate studies show a role for c-Myc in gene gating at the NPC. The c-MYC interacting protein partners at the NPC are still unknown and could potentially contribute to oncogenic responses governed by c-Myc. We utilized a Rapid Immunoprecipitation Mass spectrometry of Endogenous proteins (RIME) experiment to identify proteins, which interact with C-Myc (N-262 Abcam), TPR, and other nuclear pore proteins (Mab414). The identified protein targets from the RIME experiment were then tested in mouse embryonic fibroblasts, pancreatic cancer, and lymphoma cell lines. Currently, we are examining the role of c-Myc and RIME-identified protein interactions to uncover potential therapeutic targets in PDAC.
DO  - 10.6083/bn999752k
DO  - DOI
AB  - In recent studies, c-Myc has been shown to localize to the nuclear pore complex (NPC) after specific post-translational modifications. The localization of c-Myc to the NPC can mediate aspects of stress response to environmental signals, and separate studies show a role for c-Myc in gene gating at the NPC. The c-MYC interacting protein partners at the NPC are still unknown and could potentially contribute to oncogenic responses governed by c-Myc. We utilized a Rapid Immunoprecipitation Mass spectrometry of Endogenous proteins (RIME) experiment to identify proteins, which interact with C-Myc (N-262 Abcam), TPR, and other nuclear pore proteins (Mab414). The identified protein targets from the RIME experiment were then tested in mouse embryonic fibroblasts, pancreatic cancer, and lymphoma cell lines. Currently, we are examining the role of c-Myc and RIME-identified protein interactions to uncover potential therapeutic targets in PDAC.
AD  - Oregon Health and Science University
AD  - Oregon Health and Science University
AD  - Oregon Health and Science University
T1  - Novel protein-protein interactions with c-Myc at the nuclear pore basket
DA  - 2020
AU  - Dewson, Gabrielle
AU  - Cohn, Gabriel
AU  - Su, Yulong
L1  - https://digitalcollections.ohsu.edu/record/8434/files/ResearchWeek.2020.Dewson.Gabrielle.pdf
PB  - Oregon Health and Science University
LA  - eng
PY  - 2020
ID  - 8434
L4  - https://digitalcollections.ohsu.edu/record/8434/files/ResearchWeek.2020.Dewson.Gabrielle.pdf
KW  - Genes, myc
KW  - Nuclear Pore Complex Proteins
KW  - c-Myc
KW  - PDAC
KW  - RIME
KW  - oncology
TI  - Novel protein-protein interactions with c-Myc at the nuclear pore basket
Y1  - 2020
L2  - https://digitalcollections.ohsu.edu/record/8434/files/ResearchWeek.2020.Dewson.Gabrielle.pdf
LK  - https://digitalcollections.ohsu.edu/record/8434/files/ResearchWeek.2020.Dewson.Gabrielle.pdf
UR  - https://digitalcollections.ohsu.edu/record/8434/files/ResearchWeek.2020.Dewson.Gabrielle.pdf
ER  -