000000851 001__ 851
000000851 005__ 20251229130117.0
000000851 0247_ $$2DOI$$a10.6083/M4NG4NNF
000000851 037__ $$aETD
000000851 245__ $$aPurification and structural analysis of the cannabinoid receptor CB1: insights into allosteric regulation of a GPCR
000000851 260__ $$bOregon Health and Science University
000000851 269__ $$a2012
000000851 336__ $$aDissertation
000000851 502__ $$bPh.D.
000000851 520__ $$aThis dissertation investigates mechanisms of G protein-coupled receptor (GPCR) activation using reductionist approaches, focusing on the cannabinoid receptor CB1. Novel techniques were developed to purify CB1 and study dynamic structural changes via fluorescence spectroscopy, including the effects of an allosteric ligand. Additional work examined the structure and role of the receptor’s long N-terminus and generated CB1-specific antibodies, one of which is conformationally sensitive and modulates receptor activity. These findings advance understanding of GPCR structure-function relationships and provide tools for probing allosteric modulation and biased signaling in cannabinoid pharmacology.
000000851 540__ $$fCC BY
000000851 542__ $$fIn copyright - single owner
000000851 650__ $$aAllosteric Regulation$$014463
000000851 650__ $$aCannabinoids$$016024
000000851 650__ $$aReceptors, G-Protein-Coupled$$035314
000000851 650__ $$aStructure-Activity Relationship$$026484
000000851 650__ $$aSpectrometry, Fluorescence$$026216
000000851 650__ $$aReceptor, Cannabinoid, CB1$$035348
000000851 691__ $$aSchool of Medicine$$041369
000000851 692__ $$aDepartment of Biochemistry and Molecular Biology$$041396
000000851 7001_ $$aFay, Jonathan$$uOregon Health and Science University$$041354
000000851 7201_ $$aFarrens, David$$uOregon Health and Science University$$041354$$7Personal$$eAdvisor$$eMentor
000000851 8564_ $$9b914a0ab-2a3a-4b63-8d6a-0b10e06dcf03$$s8142163$$uhttps://digitalcollections.ohsu.edu/record/851/files/854_etd.pdf$$ePublic$$2b4441803f695dec02cb909ad9766ea1a$$31
000000851 905__ $$a/rest/prod/zs/25/x8/52/zs25x852h
000000851 909CO $$ooai:digitalcollections.ohsu.edu:851$$pstudent-work
000000851 980__ $$aTheses and Dissertations