000000854 001__ 854 000000854 005__ 20250424232551.0 000000854 0247_ $$2DOI$$a10.6083/M4862DFX 000000854 037__ $$aETD 000000854 245__ $$aIdentification of miRNA-targeted cellular pathways in flavivirus-infected cells 000000854 260__ $$bOregon Health and Science University 000000854 269__ $$a2012 000000854 336__ $$aThesis 000000854 502__ $$bM.S. 000000854 520__ $$aMicroRNAs (miRNAs) are small non-coding RNAs that are responsible for post-transcriptional gene silencing.These miRNAs are associated with the RISC (RNA-Induced Silencing Complex) that uses a seed sequence to target specific genes. Both expression of miRNAs and genes within virus-infected cells provide independent data in identifying biological hypotheses concerning regulation changes during infection. To do this, three data types are available for integration: gene expression microarray, miRNA expression microarray and RISC-Immunoprecipitation (RISC-IP) microarray. RISC-IP data provides a look at what genes are associated with the complex during infection versus uninfected cells (mock). Incorporating three independent data types allows a more complete representation of the host response to Flavivirus infection which is key to identifying miRNA regulators. This thesis integrates all three types of data for Flavivirus-infected cells using the statistical programming environment R to identify statistically enriched miRNAs regulating host response. 000000854 540__ $$fCC BY 000000854 542__ $$fIn copyright - single owner 000000854 650__ $$aMicroRNAs$$034671 000000854 650__ $$aGene Regulatory Networks$$037326 000000854 650__ $$aDengue Virus$$017471 000000854 650__ $$aComputational Biology$$031511 000000854 650__ $$aWest Nile virus$$027964 000000854 650__ $$aFlavivirus Infections$$030573 000000854 691__ $$aSchool of Medicine$$041369 000000854 692__ $$aDepartment of Medical Informatics and Clinical Epidemiology$$041422 000000854 7001_ $$aMcCoy, Chantel$$uOregon Health and Science University$$041354 000000854 7201_ $$aBankhead, Armand$$uOregon Health and Science University$$041354$$7Personal$$eAdvisor 000000854 8564_ $$9cc1ca0e5-08df-4544-994b-73636dda3ea3$$s958029$$uhttps://digitalcollections.ohsu.edu/record/854/files/857_etd.pdf$$ePublic$$26822f20776818d06ae0aeabca7033be6$$31 000000854 905__ $$a/rest/prod/d5/04/rk/38/d504rk38j 000000854 909CO $$ooai:digitalcollections.ohsu.edu:854$$pstudent-work 000000854 980__ $$aBiomedical Informatics