000000862 001__ 862
000000862 005__ 20251205111618.0
000000862 0247_ $$2DOI$$a10.6083/M4765CB0
000000862 037__ $$aETD
000000862 245__ $$aCRISPR-cas, a prokaryotic adaptive immune system, in endodontic, oral, and multidrug-resistant hospital acquired Enterococcus faecalis
000000862 260__ $$bOregon Health and Science University
000000862 269__ $$a2013
000000862 336__ $$aThesis
000000862 502__ $$bM.S.
000000862 502__ $$gEndodontics
000000862 520__ $$aCRISPR-Cas systems provide adaptive immunity against mobile genetic elements in prokaryotes. This study investigated the occurrence of CRISPR1-cas and CRISPR3-cas in 78 Enterococcus faecalis isolates from endodontic (n=34), oral (n=21), and multidrug-resistant hospital-acquired (n=23) sources, and examined associations with antibiotic resistance and virulence traits. PCR and sequencing were used to detect CRISPR-cas determinants, and phenotypic traits were assessed. CRISPR-cas was significantly more prevalent in endodontic (73%) and oral (71%) strains than in hospital-acquired strains (39%) (P=0.01 and 0.04). Absence of CRISPR-cas correlated with antibiotic resistance (P=0.04) and bacteriocin activity in endodontic strains (P=0.01). Most strains expressing virulence traits carried corresponding gene determinants. These findings suggest that CRISPR-cas may influence genetic exchange and virulence in polymicrobial endodontic environments, with implications for antimicrobial strategies in root canal therapy.
000000862 540__ $$fCC BY
000000862 542__ $$fIn copyright - single owner
000000862 650__ $$aDrug Resistance, Bacterial$$033154
000000862 650__ $$aEndodontics$$018420
000000862 650__ $$aCross Infection$$017204
000000862 650__ $$aMicrobiology$$022259
000000862 650__ $$aEnterococcus faecalis$$026448
000000862 650__ $$aRoot Canal Therapy$$025600
000000862 650__ $$aDrug Resistance, Microbial$$018080
000000862 650__ $$aCRISPR-Cas Systems$$040316
000000862 691__ $$aSchool of Dentistry$$041368
000000862 692__ $$aDepartment of Endodontology$$041413
000000862 7001_ $$aBurley, Katie$$uOregon Health and Science University$$041354
000000862 7201_ $$aSedgley, Christine$$uOregon Health and Science University$$041354$$7Personal$$eMentor
000000862 8564_ $$9275ccd0a-2244-4cfc-9d05-ce8655639bd9$$s3586560$$uhttps://digitalcollections.ohsu.edu/record/862/files/865_etd.pdf$$ePublic$$2fce29d5b8eae926e201c8858f924205b$$31
000000862 905__ $$a/rest/prod/cn/69/m4/19/cn69m4196
000000862 909CO $$ooai:digitalcollections.ohsu.edu:862$$pstudent-work
000000862 980__ $$aTheses and Dissertations