000000863 001__ 863
000000863 005__ 20260120144839.0
000000863 0247_ $$2DOI$$a10.6083/M43F4MNQ
000000863 037__ $$aETD
000000863 245__ $$aStructural plasticity and dynamic selectivity of acid-sensing ion channel - toxin complexes
000000863 260__ $$bOregon Health and Science University
000000863 269__ $$a2012
000000863 336__ $$aThesis
000000863 502__ $$bPh.D.
000000863 502__ $$gNeuroscience
000000863 520__ $$aAcid-sensing ion channels (ASICs), initially thought to gate solely via proton binding, have been shown to activate through pH-independent mechanisms, revealing diverse roles in the nervous system. To stabilize activated states, we determined crystal structures of ASIC1a bound to spider toxin PcTx1 and snake toxin MitTx. PcTx1 binds near the acidic pocket, while MitTx wedges between subunits, inducing distinct conformational changes. Structural comparisons highlight conserved scaffolds and dynamic regions that mediate gating and ion selectivity. These findings elucidate toxin-binding mechanisms and channel activation, providing a foundation for therapeutic targeting of ASICs.
000000863 540__ $$fCC BY
000000863 542__ $$fIn copyright - single owner
000000863 650__ $$aIon Channels$$021013
000000863 650__ $$aMembrane Proteins$$022009
000000863 650__ $$aCrystallography$$017234
000000863 650__ $$aVenoms$$027764
000000863 650__ $$aAcid Sensing Ion Channels$$040087
000000863 650__ $$aIon Channel Gating$$028538
000000863 650__ $$aSnake Venoms$$026086
000000863 650__ $$aCrystallography, X-Ray$$030746
000000863 691__ $$aSchool of Medicine$$041369
000000863 692__ $$aVollum Institute$$041509
000000863 7001_ $$aBaconguis, Isabelle$$uOregon Health and Science University$$041354
000000863 7201_ $$aGouaux, Eric$$uOregon Health and Science University$$041354$$7Personal$$eAdvisor
000000863 8564_ $$9f1a2bfdc-63bc-449a-bad6-cf4f729cbe48$$s4429046$$uhttps://digitalcollections.ohsu.edu/record/863/files/866_etd.pdf$$ePublic$$2ccab5343295eef5421614fa51c628ab4$$31
000000863 905__ $$a/rest/prod/xd/07/gs/75/xd07gs75z
000000863 909CO $$ooai:digitalcollections.ohsu.edu:863$$pstudent-work
000000863 956__ $$aGet Accessible Copy$$uhttps://ohsu.libwizard.com/f/requestaccessibledocument
000000863 980__ $$aTheses and Dissertations