000000878 001__ 878
000000878 005__ 20260102082254.0
000000878 0247_ $$2DOI$$a10.6083/M45B00G3
000000878 037__ $$aETD
000000878 245__ $$aTwo spatially and temporally distinct sources of calcium contribute to vesicle release at the zebrafish neuromuscular junction
000000878 260__ $$bOregon Health and Science University
000000878 269__ $$a2012
000000878 336__ $$aDissertation
000000878 502__ $$bPh.D.
000000878 520__ $$aThe zebrafish neuromuscular junction offers a unique system for studying synaptic transmission using electrophysiology and optical techniques. This work investigates two modes of vesicle release—synchronous and asynchronous—long recognized in classic neuromuscular models. Using paired recordings and pharmacological isolation, we characterized asynchronous release and its dependence on calcium dynamics. Optical imaging revealed that delayed, persistent asynchronous transmission is linked to calcium signals in motor neurons and sensitivity to the slow chelator EGTA. These findings provide mechanistic insight into asynchronous release and highlight the zebrafish neuromuscular junction as a powerful model for synaptic physiology.
000000878 540__ $$fCC BY
000000878 542__ $$fIn copyright - single owner
000000878 650__ $$aSynaptic Transmission$$022820
000000878 650__ $$aZebrafish$$028076
000000878 650__ $$aCalcium$$015963
000000878 650__ $$aNeuromuscular Junction$$022853
000000878 650__ $$aCalcium Signaling$$032061
000000878 650__ $$aExocytosis$$018776
000000878 6531_ $$azebra danio
000000878 6531_ $$aneural transmission
000000878 6531_ $$amyoneural junction
000000878 691__ $$aSchool of Medicine$$041369
000000878 7001_ $$aHubbard, Jeffrey$$uOregon Health and Science University$$041354
000000878 7201_ $$aBrehm, Paul$$uOregon Health and Science University$$041354$$7Personal$$eAdvisor
000000878 8564_ $$91c310344-2aeb-4886-b693-e83ac8c5e0b6$$s5371146$$uhttps://digitalcollections.ohsu.edu/record/878/files/881_etd.pdf$$ePublic$$2bf842cc15292b2fc6a48198f674e3441$$31
000000878 905__ $$a/rest/prod/h7/02/q6/38/h702q6382
000000878 909CO $$ooai:digitalcollections.ohsu.edu:878$$pstudent-work
000000878 980__ $$aTheses and Dissertations