000000879 001__ 879
000000879 005__ 20251216172326.0
000000879 0247_ $$2DOI$$a10.6083/M41J97S8
000000879 037__ $$aETD
000000879 245__ $$aIn search of opioid sensitive GABA afferents to midbrain dopamine neurons
000000879 260__ $$bOregon Health and Science University
000000879 269__ $$a2012
000000879 336__ $$aDissertation
000000879 502__ $$bPh.D.
000000879 502__ $$gNeuroscience
000000879 520__ $$aDopamine neurons in the ventral tegmental area (VTA) and substantia nigra regulate motivation and reward, and their disinhibition by opioids is a critical step in addiction. Using optogenetics and whole-cell recordings, we examined opioid sensitivity of GABA inputs to midbrain dopamine neurons from VTA interneurons, nucleus accumbens (NAc), and rostromedial tegmental nucleus (RMTg). RMTg inputs showed strong μ-opioid receptor-mediated inhibition, NAc inputs moderate inhibition, and VTA interneurons minimal inhibition. Striatal inputs exhibited mixed μ- and κ-opioid sensitivity. These findings identify RMTg as a major source of opioid-sensitive inhibition, providing insight into mechanisms underlying opioid-induced dopamine activation.
000000879 540__ $$fCC BY
000000879 542__ $$fIn copyright - single owner
000000879 650__ $$aMethadone$$022132
000000879 650__ $$aMesencephalon$$022077
000000879 650__ $$aVentral Tegmental Area$$030064
000000879 650__ $$aRhodopsin$$025463
000000879 650__ $$agamma-Aminobutyric Acid$$019337
000000879 650__ $$aDopaminergic Neurons$$039537
000000879 650__ $$aReceptors, Opioid, mu$$029979
000000879 6531_ $$amethadone hydrochloride
000000879 691__ $$aSchool of Medicine$$041369
000000879 692__ $$aVollum Institute$$041509
000000879 7001_ $$aMatsui, Aya$$uOregon Health and Science University$$041354
000000879 7201_ $$aWilliams, John$$uOregon Health and Science University$$041354$$7Personal$$eMentor
000000879 8564_ $$9db88d6b4-1ccc-4d6a-8f89-b59075481b78$$s1350189$$uhttps://digitalcollections.ohsu.edu/record/879/files/882_etd.pdf$$ePublic$$2f4a68f89bfa733d30da9c250c0747f53$$31
000000879 905__ $$a/rest/prod/66/82/x3/95/6682x395j
000000879 909CO $$ooai:digitalcollections.ohsu.edu:879$$pstudent-work
000000879 980__ $$aTheses and Dissertations