TY  - GEN
AB  - Zebrafish serve as a key model for auditory and vestibular research. We developed a novel method to quantify vestibular defects in larvae using the vestibulo-ocular reflex, demonstrating functional eye movements by 72 hours post-fertilization dependent on anterior/utricular otolith organs. Investigating synaptic mechanisms, we identified a point mutation (nsfI209N) that impairs synaptic transmission without altering ribbon synapse structure, while nsf null mutations cause synaptic degeneration. Nsf activity is essential for ribbon synapse maintenance, and Vamp2/Vamp8 were detected in hair cells, suggesting non-conventional SNARE-mediated transmission. Additionally, a yeast two-hybrid screen identified Tmc2a as a candidate mechanotransduction partner for Pcdh15a, warranting further study.
AD  - Oregon Health and Science University
AU  - Mo, Weike
DA  - 2013
DO  - 10.6083/M4S180H2
DO  - DOI
ED  - Nicolson, Teresa
ED  - Advisor
ID  - 881
KW  - Hearing
KW  - Zebrafish
KW  - Synapses
KW  - Mechanotransduction, Cellular
KW  - Hair Cells, Auditory
KW  - zebra danio
KW  - cellular signal transduction
L1  - https://digitalcollections.ohsu.edu/record/881/files/884_etd.pdf
L2  - https://digitalcollections.ohsu.edu/record/881/files/884_etd.pdf
L4  - https://digitalcollections.ohsu.edu/record/881/files/884_etd.pdf
LK  - https://digitalcollections.ohsu.edu/record/881/files/884_etd.pdf
N2  - Zebrafish serve as a key model for auditory and vestibular research. We developed a novel method to quantify vestibular defects in larvae using the vestibulo-ocular reflex, demonstrating functional eye movements by 72 hours post-fertilization dependent on anterior/utricular otolith organs. Investigating synaptic mechanisms, we identified a point mutation (nsfI209N) that impairs synaptic transmission without altering ribbon synapse structure, while nsf null mutations cause synaptic degeneration. Nsf activity is essential for ribbon synapse maintenance, and Vamp2/Vamp8 were detected in hair cells, suggesting non-conventional SNARE-mediated transmission. Additionally, a yeast two-hybrid screen identified Tmc2a as a candidate mechanotransduction partner for Pcdh15a, warranting further study.
PB  - Oregon Health and Science University
PY  - 2013
T1  - Hearing in zebrafish: from mechanotransduction to synaptic transmission
TI  - Hearing in zebrafish: from mechanotransduction to synaptic transmission
UR  - https://digitalcollections.ohsu.edu/record/881/files/884_etd.pdf
Y1  - 2013
ER  -