000000881 001__ 881
000000881 005__ 20251205134651.0
000000881 0247_ $$2DOI$$a10.6083/M4S180H2
000000881 037__ $$aETD
000000881 245__ $$aHearing in zebrafish: from mechanotransduction to synaptic transmission
000000881 260__ $$bOregon Health and Science University
000000881 269__ $$a2013
000000881 336__ $$aDissertation
000000881 502__ $$bPh.D.
000000881 502__ $$gCell & Developmental Biology (sunsetting)
000000881 520__ $$aZebrafish serve as a key model for auditory and vestibular research. We developed a novel method to quantify vestibular defects in larvae using the vestibulo-ocular reflex, demonstrating functional eye movements by 72 hours post-fertilization dependent on anterior/utricular otolith organs. Investigating synaptic mechanisms, we identified a point mutation (nsfI209N) that impairs synaptic transmission without altering ribbon synapse structure, while nsf null mutations cause synaptic degeneration. Nsf activity is essential for ribbon synapse maintenance, and Vamp2/Vamp8 were detected in hair cells, suggesting non-conventional SNARE-mediated transmission. Additionally, a yeast two-hybrid screen identified Tmc2a as a candidate mechanotransduction partner for Pcdh15a, warranting further study.
000000881 540__ $$fCC BY
000000881 542__ $$fIn copyright - single owner
000000881 650__ $$aHearing$$019916
000000881 650__ $$aZebrafish$$028076
000000881 650__ $$aSynapses$$026704
000000881 650__ $$aMechanotransduction, Cellular$$035047
000000881 650__ $$aHair Cells, Auditory$$019810
000000881 6531_ $$azebra danio
000000881 6531_ $$acellular signal transduction
000000881 691__ $$aSchool of Medicine$$041369
000000881 692__ $$aDepartment of Cell, Developmental and Cancer Biology$$041399
000000881 7001_ $$aMo, Weike$$uOregon Health and Science University$$041354
000000881 7201_ $$aNicolson, Teresa$$uOregon Health and Science University$$041354$$7Personal$$eAdvisor
000000881 8564_ $$98702ee33-8ea6-4803-bc36-d9b64e07e437$$s4762718$$uhttps://digitalcollections.ohsu.edu/record/881/files/884_etd.pdf$$ePublic$$20453911908bf51a9f1ff831d5d75dbea$$31
000000881 905__ $$a/rest/prod/ws/85/9f/69/ws859f695
000000881 909CO $$ooai:digitalcollections.ohsu.edu:881$$pstudent-work
000000881 980__ $$aTheses and Dissertations