000000884 001__ 884
000000884 005__ 20260120144840.0
000000884 0247_ $$2DOI$$a10.6083/M4CV4FRM
000000884 037__ $$aETD
000000884 245__ $$aHIV vaccine design based on in vivo evolution of quasispecies envelope proteins
000000884 260__ $$bOregon Health and Science University
000000884 269__ $$a2012
000000884 336__ $$aDissertation
000000884 502__ $$bPh.D.
000000884 520__ $$aBroadly neutralizing antibodies (bNAbs) against HIV remain a key goal for vaccine development. This work investigates whether sequential immunization with Env variants derived from HIV-infected individuals who developed bNAbs can guide B cell maturation toward breadth. Using phylogenetic analysis of viral quasispecies and antigenic profiling, we selected Env immunogens and tested DNA-plus-protein co-immunization strategies in rabbits. Results suggest that increasing Env sequence divergence and combining DNA with recombinant gp140 protein enhances neutralization responses compared to protein alone. These findings provide insight into antigenicity-immunogenicity relationships and inform rational design of HIV vaccines aimed at eliciting bNAbs.
000000884 540__ $$fCC BY
000000884 542__ $$fIn copyright - single owner
000000884 650__ $$aAIDS Vaccines$$029526
000000884 650__ $$aImmunoglobulins$$020695
000000884 650__ $$aRabbits$$025058
000000884 650__ $$aAntibodies$$014849
000000884 650__ $$aHIV Infections$$028555
000000884 650__ $$aVaccine Development$$013568
000000884 650__ $$aQuasispecies$$012396
000000884 650__ $$aBroadly Neutralizing Antibodies$$013185
000000884 650__ $$aHIV$$020258
000000884 650__ $$aenv Gene Products, Human Immunodeficiency Virus$$037739
000000884 650__ $$aAntibodies, Neutralizing$$038855
000000884 691__ $$aSchool of Medicine$$041369
000000884 692__ $$aDepartment of Molecular Microbiology and Immunology$$041429
000000884 7001_ $$aPissani, Franco$$uOregon Health and Science University$$041354
000000884 7201_ $$aHaigwood, Nancy$$uOregon Health and Science University$$041354$$7Personal$$eAdvisor
000000884 8564_ $$91382b364-4672-43da-9130-d1e2ed012423$$s3035729$$uhttps://digitalcollections.ohsu.edu/record/884/files/887_etd.pdf$$ePublic$$23dfc1ee1ba2c499d9c358608c8878aa3$$31
000000884 905__ $$a/rest/prod/p8/41/8n/25/p8418n25z
000000884 909CO $$ooai:digitalcollections.ohsu.edu:884$$pstudent-work
000000884 956__ $$aGet Accessible Copy$$uhttps://ohsu.libwizard.com/f/requestaccessibledocument
000000884 980__ $$aTheses and Dissertations