000008977 001__ 8977
000008977 005__ 20250424233421.0
000008977 0247_ $$2DOI$$a10.6083/M4RF5S2F
000008977 037__ $$aETD
000008977 245__ $$aSite directed mutagenesis of residue lysine 157 of the hypoxanthine-guanine phosphoribosyltransferase gene from Leishmania donovani and kinetic analysis and comparison of site directed mutants with the recombinant wild type enzyme
000008977 260__ $$bOregon Health and Science University
000008977 269__ $$a2004-06-01
000008977 336__ $$aThesis
000008977 502__ $$bM.S.
000008977 520__ $$aParasitic protozoa, which are auxotrophic for purines, have evolved a unique metabolic pathway, the purine salvage pathway, to meet their requirements for purines. Since they are unable to synthesize their purines, they must salvage these essential nutrients from their hosts. Numerous enzymes are involved in allowing the parasitic protozoa to obtain the purines it needs from its host, the enzyme hypoxanthine-guanine phosphoribosyltransferase (HGPRT). Appears to be a key enzyme in purine salvage Salvage used by these parasites. This thesis examines the role of the active site residue lysine 157 within the HGPRT of Leishmania donovani.
000008977 542__ $$fIn copyright - single owner
000008977 650__ $$aHypoxanthine Phosphoribosyltransferase$$020605
000008977 650__ $$aLeishmania donovani$$021391
000008977 650__ $$aPurines$$024936
000008977 691__ $$aSchool of Medicine$$041369
000008977 692__ $$aDepartment of Biochemistry and Molecular Biology$$041396
000008977 7001_ $$aBarton, Gregory$$uOregon Health and Science University$$041354
000008977 7201_ $$aUllman, Buddy$$uOregon Health and Science University$$041354$$7Personal$$eAdvisor
000008977 8564_ $$95ec5c555-bed6-4a90-884b-1e7a8a79cb0f$$s6503750$$uhttps://digitalcollections.ohsu.edu/record/8977/files/2004.barton.gregory.pdf$$ePublic$$23ec0b39456410872b813ad99cd03f861$$31
000008977 905__ $$a/rest/prod/xs/55/mc/77/xs55mc77q
000008977 909CO $$ooai:digitalcollections.ohsu.edu:8977$$pstudent-work
000008977 980__ $$aTheses and Dissertations