TY  - GEN
N2  - This thesis work highlights development and applications for both circulating extracellular vesicles (EV) and cell-free RNA (cf-RNA) toward liquid biopsy based early cancer detection methodologies. Effective detection and monitoring for signatures of oncological disease in a noninvasive manner are urgently needed to reduce the morbidity and mortality caused by cancer. Circulating EVs and cf-RNA are intensely sought after biomarkers in liquid biopsy. Their roles in cell-to-cell communication, ability to reflect phenotypic changes from cells, and tissues of origin are becoming better understood. Despite this, current literatures present key challenges which limit the promise of liquid biopsy based early cancer detection: i) there is a lack of standardized blood processing for multi-omics which minimizes ex-vivo processing artefacts via discerning true in-vivo signatures from ex-vivo artefacts; ii) daily fluctuations and the influence of meal consumption on EV and cf-RNA levels are not clear; iii) a comprehensive study of cf-RNA for cancer detection, pan cancer discernment, and high risk group identification has not been conducted; and iv) the selective packaging of cf-RNA carriers and its association with cancer are unknown.
DO  - 10.6083/xs55mc819
DO  - DOI
AB  - This thesis work highlights development and applications for both circulating extracellular vesicles (EV) and cell-free RNA (cf-RNA) toward liquid biopsy based early cancer detection methodologies. Effective detection and monitoring for signatures of oncological disease in a noninvasive manner are urgently needed to reduce the morbidity and mortality caused by cancer. Circulating EVs and cf-RNA are intensely sought after biomarkers in liquid biopsy. Their roles in cell-to-cell communication, ability to reflect phenotypic changes from cells, and tissues of origin are becoming better understood. Despite this, current literatures present key challenges which limit the promise of liquid biopsy based early cancer detection: i) there is a lack of standardized blood processing for multi-omics which minimizes ex-vivo processing artefacts via discerning true in-vivo signatures from ex-vivo artefacts; ii) daily fluctuations and the influence of meal consumption on EV and cf-RNA levels are not clear; iii) a comprehensive study of cf-RNA for cancer detection, pan cancer discernment, and high risk group identification has not been conducted; and iv) the selective packaging of cf-RNA carriers and its association with cancer are unknown.
T1  - Discovery of early cancer signatures in circulating RNA and extracellular vesicles
DA  - 2021
AU  - Kim, Hyun J.
L1  - https://digitalcollections.ohsu.edu/record/9098/files/Kim.Hyun.Ji.2021.pdf
PB  - Oregon Health and Science University
PY  - 2021
ID  - 9098
L4  - https://digitalcollections.ohsu.edu/record/9098/files/Kim.Hyun.Ji.2021.pdf
KW  - Extracellular Vesicles
KW  - Neoplasms
KW  - Biomarkers
KW  - Liquid Biopsy
KW  - Cell-Free Nucleic Acids
KW  - messenger rna
TI  - Discovery of early cancer signatures in circulating RNA and extracellular vesicles
Y1  - 2021
L2  - https://digitalcollections.ohsu.edu/record/9098/files/Kim.Hyun.Ji.2021.pdf
LK  - https://digitalcollections.ohsu.edu/record/9098/files/Kim.Hyun.Ji.2021.pdf
UR  - https://digitalcollections.ohsu.edu/record/9098/files/Kim.Hyun.Ji.2021.pdf
ER  -