000009098 001__ 9098
000009098 005__ 20240124114317.0
000009098 0247_ $$2DOI$$a10.6083/xs55mc819
000009098 037__ $$aETD
000009098 245__ $$aDiscovery of early cancer signatures in circulating RNA and extracellular vesicles
000009098 260__ $$bOregon Health and Science University
000009098 269__ $$a2021
000009098 336__ $$aDissertation
000009098 502__ $$bPh.D.
000009098 520__ $$aThis thesis work highlights development and applications for both circulating extracellular vesicles (EV) and cell-free RNA (cf-RNA) toward liquid biopsy based early cancer detection methodologies. Effective detection and monitoring for signatures of oncological disease in a noninvasive manner are urgently needed to reduce the morbidity and mortality caused by cancer. Circulating EVs and cf-RNA are intensely sought after biomarkers in liquid biopsy. Their roles in cell-to-cell communication, ability to reflect phenotypic changes from cells, and tissues of origin are becoming better understood. Despite this, current literatures present key challenges which limit the promise of liquid biopsy based early cancer detection: i) there is a lack of standardized blood processing for multi-omics which minimizes ex-vivo processing artefacts via discerning true in-vivo signatures from ex-vivo artefacts; ii) daily fluctuations and the influence of meal consumption on EV and cf-RNA levels are not clear; iii) a comprehensive study of cf-RNA for cancer detection, pan cancer discernment, and high risk group identification has not been conducted; and iv) the selective packaging of cf-RNA carriers and its association with cancer are unknown.
000009098 542__ $$fIn copyright - single owner
000009098 650__ $$aExtracellular Vesicles$$011111
000009098 650__ $$aNeoplasms$$022758
000009098 650__ $$aBiomarkers$$028395
000009098 650__ $$aLiquid Biopsy$$012229
000009098 650__ $$aCell-Free Nucleic Acids$$012228
000009098 6531_ $$amessenger rna
000009098 691__ $$aSchool of Medicine$$041369
000009098 692__ $$aDepartment of Biomedical Engineering$$041397
000009098 7001_ $$aKim, Hyun J.
000009098 8564_ $$98b4f80b7-dc14-46a4-8be0-58a15cf0b33b$$s11591713$$uhttps://digitalcollections.ohsu.edu/record/9098/files/Kim.Hyun.Ji.2021.pdf
000009098 905__ $$a/rest/prod/xs/55/mc/81/xs55mc819
000009098 909CO $$ooai:digitalcollections.ohsu.edu:9098$$pstudent-work
000009098 980__ $$aTheses and Dissertations