TY  - GEN
N2  - This dissertation describes the development of the TRADE system for directed evolution of the AAV capsid, its successful application to identify the HN1 variant, a novel AAV capsid with enhanced neuronal transduction efficiency and specificity following intravenous vector administration in both rodents and rhesus macaques. This work establishes a novel technology with the potential to facilitate the rapid development of customized AAV vectors targeting cell types of choice and has the potential to significantly impact a wide variety of problems in basic science and medicine where lack of an efficient vector for targeting a critical cell type poses a significant barrier. Thus, TRADE is poised to transform the field of AAV capsid directed evolution.
DO  - 10.6083/cz30pt42j
DO  - DOI
AB  - This dissertation describes the development of the TRADE system for directed evolution of the AAV capsid, its successful application to identify the HN1 variant, a novel AAV capsid with enhanced neuronal transduction efficiency and specificity following intravenous vector administration in both rodents and rhesus macaques. This work establishes a novel technology with the potential to facilitate the rapid development of customized AAV vectors targeting cell types of choice and has the potential to significantly impact a wide variety of problems in basic science and medicine where lack of an efficient vector for targeting a critical cell type poses a significant barrier. Thus, TRADE is poised to transform the field of AAV capsid directed evolution.
T1  - Transcription-dependent directed evolution of the adeno-associated virus capsid
DA  - 2021
AU  - Huang, Samuel J.
L1  - https://digitalcollections.ohsu.edu/record/9136/files/Huang.Samuel.2021.pdf
PB  - Oregon Health and Science University
PY  - 2021
ID  - 9136
L4  - https://digitalcollections.ohsu.edu/record/9136/files/Huang.Samuel.2021.pdf
KW  - Dependovirus
KW  - Capsid
KW  - Genetic Therapy
KW  - Central Nervous System
KW  - adeno-associated virus
TI  - Transcription-dependent directed evolution of the adeno-associated virus capsid
Y1  - 2021
L2  - https://digitalcollections.ohsu.edu/record/9136/files/Huang.Samuel.2021.pdf
LK  - https://digitalcollections.ohsu.edu/record/9136/files/Huang.Samuel.2021.pdf
UR  - https://digitalcollections.ohsu.edu/record/9136/files/Huang.Samuel.2021.pdf
ER  -