000009136 001__ 9136
000009136 005__ 20240124114318.0
000009136 0247_ $$2DOI$$a10.6083/cz30pt42j
000009136 037__ $$aETD
000009136 245__ $$aTranscription-dependent directed evolution of the adeno-associated virus capsid
000009136 260__ $$bOregon Health and Science University
000009136 269__ $$a2021
000009136 336__ $$aDissertation
000009136 502__ $$bPh.D.
000009136 520__ $$aThis dissertation describes the development of the TRADE system for directed evolution of the AAV capsid, its successful application to identify the HN1 variant, a novel AAV capsid with enhanced neuronal transduction efficiency and specificity following intravenous vector administration in both rodents and rhesus macaques. This work establishes a novel technology with the potential to facilitate the rapid development of customized AAV vectors targeting cell types of choice and has the potential to significantly impact a wide variety of problems in basic science and medicine where lack of an efficient vector for targeting a critical cell type poses a significant barrier. Thus, TRADE is poised to transform the field of AAV capsid directed evolution.
000009136 542__ $$fIn copyright - single owner
000009136 650__ $$aDependovirus$$014224
000009136 650__ $$aCapsid$$016051
000009136 650__ $$aGenetic Therapy$$028314
000009136 650__ $$aCentral Nervous System$$016312
000009136 6531_ $$aadeno-associated virus
000009136 691__ $$aSchool of Medicine$$041369
000009136 692__ $$aDepartment of Physiology and Pharmacology$$041442
000009136 7001_ $$aHuang, Samuel J.
000009136 8564_ $$92db3dff9-3aaa-41fc-981c-7f4d934c14f6$$s17731750$$uhttps://digitalcollections.ohsu.edu/record/9136/files/Huang.Samuel.2021.pdf
000009136 905__ $$a/rest/prod/cz/30/pt/42/cz30pt42j
000009136 909CO $$ooai:digitalcollections.ohsu.edu:9136$$pstudent-work
000009136 980__ $$aTheses and Dissertations