000009292 001__ 9292
000009292 005__ 20240124114320.0
000009292 0247_ $$2DOI$$a10.6083/vh53ww43g
000009292 037__ $$aETD
000009292 245__ $$aIn situ therapeutic response imaging using a novel fluorescence imaging platform - TRIPODD
000009292 260__ $$bOregon Health and Science University
000009292 269__ $$a2021
000009292 336__ $$aDissertation
000009292 502__ $$bPh.D.
000009292 520__ $$aSuccessful cancer treatment continues to elude modern medicine and its arsenal of therapeutic strategies. Therapy resistance is driven by tumor heterogeneity, complex interactions between malignant, microenvironmental and immune cells and signaling pathway cross talk. With the ultimate goal of improved molecularly targeted therapeutic efficacy, we have developed and optimized a fluorescence imaging platform termed TRIPODD (Therapeutic Response Imaging through Proteomic and Optical Drug Distribution), resulting in the only methodology capable of simultaneous quantification of single-cell drug target availability and protein expression with preserved spatial context within a tumor. TRIPODD will enable an improved mechanistic understanding of clinically-relevant treatment regimens through spatially resolved single-cell quantification of drug concentration and proteomic response to identify mechanisms of resistant subclonal population outgrowths driving therapeutic resistance.
000009292 542__ $$fIn copyright - single owner
000009292 650__ $$aProteomics$$035059
000009292 650__ $$aHigh-Throughput Nucleotide Sequencing$$039471
000009292 650__ $$aFluorescent Antibody Technique$$019123
000009292 6531_ $$adrug therapeutic index
000009292 6531_ $$afluorescence imaging
000009292 691__ $$aSchool of Medicine$$041369
000009292 692__ $$aDepartment of Biomedical Engineering$$041397
000009292 7001_ $$aMcMahon, Nathan P.
000009292 8564_ $$990766ec5-9e14-406b-be60-1cc22bac9b56$$s4862112$$uhttps://digitalcollections.ohsu.edu/record/9292/files/McMahon.Nathan.2021.pdf
000009292 905__ $$a/rest/prod/vh/53/ww/43/vh53ww43g
000009292 909CO $$ooai:digitalcollections.ohsu.edu:9292$$pstudent-work
000009292 980__ $$aTheses and Dissertations