TY  - GEN
N2  - Prostate cancer is the second most commonly diagnosed cancer in men worldwide. This thesis leverages novel single-cell epigenomic and proteomic approaches to aid in the identification of precise subtypes of prostate tumors that are organ-confined at the time of diagnosis. We first utilize cyclic immunofluorescent imaging to profile the spatial heterogeneity of prostate tumors and uncover risk-level and Gleason Pattern specific cell types and cellular interactions. Next, we perform single-cell ATAC sequencing of the same tumors and identify unique genomic regions associated with high-risk tumors and immune response signatures associated with low-risk tumors. Finally, we develop assays that enable multiplex imaging of biomarkers isolated from low-volume liquid biopsy samples. Our work uncovers unique cellular and molecular markers, which hold the potential to become novel prognostic factors, and presents a multiplex liquid biopsy approach for the detection of prostate cancer for precise treatment strategies.
DO  - 10.6083/n583xv754
DO  - DOI
AB  - Prostate cancer is the second most commonly diagnosed cancer in men worldwide. This thesis leverages novel single-cell epigenomic and proteomic approaches to aid in the identification of precise subtypes of prostate tumors that are organ-confined at the time of diagnosis. We first utilize cyclic immunofluorescent imaging to profile the spatial heterogeneity of prostate tumors and uncover risk-level and Gleason Pattern specific cell types and cellular interactions. Next, we perform single-cell ATAC sequencing of the same tumors and identify unique genomic regions associated with high-risk tumors and immune response signatures associated with low-risk tumors. Finally, we develop assays that enable multiplex imaging of biomarkers isolated from low-volume liquid biopsy samples. Our work uncovers unique cellular and molecular markers, which hold the potential to become novel prognostic factors, and presents a multiplex liquid biopsy approach for the detection of prostate cancer for precise treatment strategies.
T1  - Multi-omic characterization of localized prostate cancer and development of multiplex liquid biopsy assays for accurate patient stratification
DA  - 2021
AU  - Sayar, Zeynep
L1  - https://digitalcollections.ohsu.edu/record/9380/files/Sayar.Zeynep.2021.pdf
PB  - Oregon Health and Science University
PY  - 2021
ID  - 9380
L4  - https://digitalcollections.ohsu.edu/record/9380/files/Sayar.Zeynep.2021.pdf
KW  - Prostatic Neoplasms
KW  - Prostate-Specific Antigen
KW  - Liquid Biopsy
KW  - Immunity
KW  - Genomics
TI  - Multi-omic characterization of localized prostate cancer and development of multiplex liquid biopsy assays for accurate patient stratification
Y1  - 2021
L2  - https://digitalcollections.ohsu.edu/record/9380/files/Sayar.Zeynep.2021.pdf
LK  - https://digitalcollections.ohsu.edu/record/9380/files/Sayar.Zeynep.2021.pdf
UR  - https://digitalcollections.ohsu.edu/record/9380/files/Sayar.Zeynep.2021.pdf
ER  -