000009380 001__ 9380
000009380 005__ 20231129124948.0
000009380 0247_ $$2DOI$$a10.6083/n583xv754
000009380 037__ $$aETD
000009380 245__ $$aMulti-omic characterization of localized prostate cancer and development of multiplex liquid biopsy assays for accurate patient stratification
000009380 260__ $$bOregon Health and Science University
000009380 269__ $$a2021
000009380 336__ $$aDissertation
000009380 502__ $$bPh.D.
000009380 520__ $$aProstate cancer is the second most commonly diagnosed cancer in men worldwide. This thesis leverages novel single-cell epigenomic and proteomic approaches to aid in the identification of precise subtypes of prostate tumors that are organ-confined at the time of diagnosis. We first utilize cyclic immunofluorescent imaging to profile the spatial heterogeneity of prostate tumors and uncover risk-level and Gleason Pattern specific cell types and cellular interactions. Next, we perform single-cell ATAC sequencing of the same tumors and identify unique genomic regions associated with high-risk tumors and immune response signatures associated with low-risk tumors. Finally, we develop assays that enable multiplex imaging of biomarkers isolated from low-volume liquid biopsy samples. Our work uncovers unique cellular and molecular markers, which hold the potential to become novel prognostic factors, and presents a multiplex liquid biopsy approach for the detection of prostate cancer for precise treatment strategies.
000009380 542__ $$fIn copyright - single owner
000009380 650__ $$aProstatic Neoplasms$$024731
000009380 650__ $$aProstate-Specific Antigen$$029960
000009380 650__ $$aLiquid Biopsy$$012229
000009380 650__ $$aImmunity$$020668
000009380 650__ $$aGenomics$$033016
000009380 691__ $$aSchool of Medicine$$041369
000009380 692__ $$aDepartment of Biomedical Engineering$$041397
000009380 7001_ $$aSayar, Zeynep
000009380 8564_ $$9fbe526d5-fbad-4c49-b9ee-cbdfe0ec43df$$s10809815$$uhttps://digitalcollections.ohsu.edu/record/9380/files/Sayar.Zeynep.2021.pdf
000009380 905__ $$a/rest/prod/n5/83/xv/75/n583xv754
000009380 909CO $$ooai:digitalcollections.ohsu.edu:9380$$pstudent-work
000009380 980__ $$aTheses and Dissertations