000009445 001__ 9445
000009445 005__ 20240124114322.0
000009445 0247_ $$2DOI$$a10.6083/mg74qm90c
000009445 037__ $$aETD
000009445 245__ $$aEngineering fluorescent protein biosensors to monitor glycolytic metabolism
000009445 260__ $$bOregon Health and Science University
000009445 269__ $$a2022
000009445 336__ $$aDissertation
000009445 502__ $$bPh.D.
000009445 520__ $$aFluorescent protein biosensors have become an important tool for neuroscience research. Highly optimized biosensors for Ca2+ are widely used to study neuronal activity in live animals. Underlying the impressive functionality of these fluorescent probes has been a tremendous research and engineering effort to understand their function and engineer new variants with improved performance. While the potential for fluorescent biosensors to enable new directions in biological research is clear, biosensors for other targets have not reached the same widespread usage or impact. Underlying this is a combination of factors including the difficulty of generating new and improved biosensors and the challenge of identifying informative analytes to measure.
000009445 542__ $$fIn copyright - single owner
000009445 650__ $$aGlycolysis$$019653
000009445 650__ $$aFluorescence$$019121
000009445 650__ $$aMetabolism$$022101
000009445 650__ $$aProtein Engineering$$028214
000009445 6531_ $$abiosensors
000009445 691__ $$aSchool of Medicine$$041369
000009445 692__ $$aVollum Institute$$041509
000009445 7001_ $$aKoberstein, John N.
000009445 8564_ $$95a8157d7-be4e-4169-b36c-0a05054d3bd7$$s4288506$$uhttps://digitalcollections.ohsu.edu/record/9445/files/Koberstein.John.2022.pdf
000009445 905__ $$a/rest/prod/mg/74/qm/90/mg74qm90c
000009445 909CO $$ooai:digitalcollections.ohsu.edu:9445$$pstudent-work
000009445 980__ $$aTheses and Dissertations