000009475 001__ 9475
000009475 005__ 20240124114322.0
000009475 0247_ $$2DOI$$a10.6083/nc580n47v
000009475 037__ $$aETD
000009475 245__ $$aExocytosis and endocytosis of synaptic vesicles is regulated by calcium
000009475 260__ $$bOregon Health and Science University
000009475 269__ $$a2022
000009475 336__ $$aDissertation
000009475 502__ $$bPh.D.
000009475 520__ $$aNeurons communicate with each other either through electrical synapses formed by gap junctions, or by the synaptic release of neurotransmitters. Release of neurotransmitter into the synaptic cleft is occurs when loaded synaptic vesicles fuse to the plasma membrane (exocytosis). Neurons have a compensatory process that retrieves fused vesicle membrane from the active zone (endocytosis), maintaining membrane homeostasis. Presynaptic capacitance (Cm) measurements can directly test exo- and endocytosis mechanisms with microsecond temporal resolution without interference from saturation or desensitization of postsynaptic receptors. The biggest limitation of Cm measurements is that they require high frequency stimulation, which has limited this technique to cells with simple morphology or patchable large terminals. Developments in this field indicate that neurons and synaptic terminals with more complicated morphology can be studied using this technique. The focus of this study is on a retinal interneuron, the AII amacrine cell (AII-AC), that is the only inhibitory interneuron to be studied using Cm measurements to date.
000009475 542__ $$fIn copyright - single owner
000009475 650__ $$aExocytosis$$018776
000009475 650__ $$aCalcium$$015963
000009475 650__ $$aEndocytosis$$018417
000009475 6531_ $$asynaptic inhibition
000009475 6531_ $$aall amacrine cell
000009475 6531_ $$aneurotransmission
000009475 691__ $$aSchool of Medicine$$041369
000009475 692__ $$aVollum Institute$$041509
000009475 7001_ $$aMeadows, Marc A.
000009475 8564_ $$9e5e9be62-3953-4978-b7d9-1d9c1a54cc43$$s11209737$$uhttps://digitalcollections.ohsu.edu/record/9475/files/Meadows.Marc.2022.pdf
000009475 905__ $$a/rest/prod/nc/58/0n/47/nc580n47v
000009475 909CO $$ooai:digitalcollections.ohsu.edu:9475$$pstudent-work
000009475 980__ $$aTheses and Dissertations